1. European Biotechnology Congress, Bucharest, Romania May 07-09, 2015
Study upon the haematological
parameters in minipig models
Ioan Huțu1, Irina Patras1,
Esra Elena Foditsh2, Reinhold Zimmermann2, Calin Mircu1*
1Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine King Mihai I of Romania Timisoara, Romania 2Spinal Cord Injury and Tissue Regeneration Centre, Paracelsus Medical University, Austria
* Corresponding author
Results and Conclusion:
The results obtained was classified as low, normal or high. Comparing the obtained values with other parameters and results of clinical examination we conclude that the classification of the equipment not necessary meets the normal status of Göttingen minipigs, as reflected in the software interpretation. Therefore, we used the reference values provided by the supplier of biological material - Elegaard Göttingen Minipigs®.
Kruskal Wallis statistical analysis performed on blood counts shows that surgical interventions and neuro- modulation had an impact on mean corpuscular volume (p<0.033), white blood cell count (neutrophils p<0.004 and basophils) and percentage of the distribution in differential leucocyte count (p<0.008) to lymphocytes (p<0,002), monocytes (p<0.020) and basophils (p<0.008).
Keywords: minipig, spinal cord injury, haematological parameters
Background:
Minipig models are used frequently in many fields of biomedical research due to the i) social acceptance, ii) existence of a large number of similarities between the swine’s and human’s physiology and iii) easy training and handling. AIM: To observe and compare the haematology dynamics during the follow-up period between four groups of minipigs, considering the type of intervention applied.
Methods:
The experiment was conducted on 21 Gottingen Minipig, adult females which underwent different surgical procedures, as follows: thoracic vertebrae laminectomy, laparoscopic intervention or both – representing the spinal cord injury model (SCI) and control group (healthy individuals). 64 blood samples were processed using an automatic Haematology analyser IDEXX model Procyte Dx™ with veterinary software for 25 parameters.
Acknowledgements: This work was done using facilities from Horia Cernescu Research Unit, in DICES-Ro05 - POSCCE 145 project.
European Biotechnology Congress, Bucharest, Romania May 07-09, 2015