1. Clinical Spectrum of Leishmaniasis
Cutaneous Leishmaniasis (CL)
most common form, relatively benign self-healing skin lesions (aka, localized or simple CL)
Mucocutaneous Leishmaniasis (MCL)
simple skin lesions that metastasize to mucosae (especially nose and mouth region)
Visceral Leishmaniasis (VL)
generalized infection of the reticuloendothelial system, high mortality

3. William Boog LEISHMAN (1865-1926)
Leishmania donovani
Causative agent of _____________________________________, or visceral leishmaniasis
Identified by William Leishman in 1900 from a soldier who died of fever in Dum-Dum, India. Charles Donovan identified the parasites in the spleen of an infected person in Parasite is named in honor of these two men.
                                        
                    
William Boog LEISHMAN ( )
Charles DONOVAN ( )

4. Amastigote: oval, 2-5 microns by 1 - 3 microns
Amastigote: oval, 2-5 microns by microns. Most likely seen inside macrophages.
Promastigote: rarely seen in clinical samples, most likely recovered from gut of sand fly: microns by  microns, similar in size & appearance to trypanosomes

5. Diagnosis geographical presence of parasite
demonstration of parasite in skin lesion or bone marrow(NNN medium)Giemsa stain
delayed hypersensitivity skin test (cutaneous forms)
serological tests
For Visceral Leishmaniasis:
enzyme liked immunosorbent assay (ELISA)
direct agglutination test (DAT)
indirect fluorescent antibody test (IFAT)

6. Treatment pentavalent antimonials (pentostam)
amphotericin B (less toxic, expensive)
miltefosine (phase IV, no hospitalization

7. Sand fly Phlebotomus & Lutzomia

8. Amastigotes

9. Promastigotes

16. Amastigoites in macrophages
macrophage from a lymph node of a dog. 
Leishman-Donovan or LD bodies). Lying in macrophage cells from liver. Giemsa

17. Diffuse cuetaneous leishmaniasis

18. macrophage filled with Leishmania amastigotes.

19. hepatosplenomegaly

20. mucocutaneous

21. NNN medium