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Laboratory Investigations

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Presentation on theme: "Laboratory Investigations"— Presentation transcript:

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2 Laboratory Investigations
Urine Analysis/ Sensitivity Hematological Profile Hemostatic Profile Biochemical Profile Serological & Immunological Profile

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4 Urine Report

5 Urine Sensitivity

6 Hematological Profile
Complete Blood Count (CBC) “Blood Film, RBC Count, Hb, HCT, Indices, RDW” Reticulocyte Percentage Ferrokinetics “Iron, TIBC, Ferritin, Soluble Transferrin Receptors” Others “Hb Electrophoresis, Sickling Test, Schilling Test, G6PD” What Type of Anemia?

7 Reference Rage Patient Result Test million 3.32 million RBC 13-18 g% 8.2 g% Hb 40-54% 24% HCT 76-96 fL 72 fL MCV 27-32 pg 25 pg MCH 30-35% 34% MCHC % 18% RDW Reference Rage Patient Result Test million 4.4 million RBC 13-18 g% 12.5 g% Hb 40-54% 37% HCT 76-96 fL 84 fL MCV 27-32 pg 28 pg MCH 30-35% 34% MCHC % 10% RDW 0.2-2% 0.83% Retics Reference Rage Patient Result Test μg% 29 μg% Iron 5-184 ng/ml 3 ng/ml Ferritin μg% 150 μg% TIBC

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9 Infection or Blood Malignancy?
Hematological Profile (Cont.) WBC Cont Neutrophils Eosinophils Basophils Monocytes Lymphocyte Immature Cells Infection or Blood Malignancy?

10 Reference Rage Patient Result Test 4-11 thousands 6.8 Thousands WBC 47-75% 79% Neutrophils, Segmented 0-5% 3% Neutrophils, Staff 20-45% 12% Lymphocytes 3-8% 4% Monocytes 0-6% 2% Eosinophils 0-1% 0% Basophils

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12 Clotting Factors Assay Antiplatelet Antibody Platelet Aggregation Test
Hemostatic Profile Bleeding Hyper-Coagurable State or Antithrombine III Protein C Protein S D-Dimer Clotting Factors Assay APTT PT, INR, Activity Antiplatelet Antibody Platelet Aggregation Test Platelets Count

13 Reference Rage Patient Result Test 90000 Platelets Count

14 Biochemistry Report Kidney Function Tests Liver Function Tests
Heart Function Tests Lipid Profile Hormonal Profile Tumor Markers Diabetic Profile Osteoporosis Profile

15 Albumin/Creatinine Ratio (ACR)
Kidney Function Tests Serum Creatinine Albumin/Creatinine Ratio (ACR) BUN Uric Acid Na K

16 Reference Rage Patient Result Test 10-45 mg% 105 mg% BUN 0.5-1.5 mg%
Serum Creatinine mg% 9.8 mg% Serum Uric Acid Microalbuminuria: ACR Macroalbuminuria: ACR >300

17 Liver Function Tests AST ALT ALP GGT Albumin T. & D. Bilirubin
HCC AST ALT ALP GGT Albumin T. & D. Bilirubin Hepatitis Markers

18 Reference Rage Patient Result Test mg% 15.7 mg% T. Bil Up to 0.4 mg% 4 mg% D. Bil Up to 40 U/L 204 U/L sAST 193 U/L sALT U/L 495 U/L ALP g% 5.2 g% Albumin g% 2.4 g% Globulin >1 2.17 A/G Ratio

19 Cut off Value Patient Result Test 0.075 mIU/L 0.021 mIU/L HBsAg 0.23 mIU/L 0.098 mIU/L HCV Ab 0.075 mU/ml >100 mU/ml HAV

20 Fibro Test measures the hepatic fibrosis stage with a blood test.
Minimal Moderate Severe

21 Acti Test measures the degree of necroinflammatory activity of viral origin (HBV and HCV).
Minimal Moderate Severe

22 Heart Function Tests Troponin CK-Mb AST LDH

23 Reference Rage Patient Result Test 0-25 U/L 35 U/L CK-Mb U/L 490 U/L LDH 0-0.2 ng/ml 0.8 ng/ml Troponin-I

24 Lipids Profile Total Cholesterol LDL-C HDL-C VLDL-C Atherogenic Inex
Triacylglycerol Phospholipids

25 Reference Rage Patient Result Test Normal <200 mg% 300 mg% T. Cholesterol Borderline mg% High >240 mg% mg% 180 mg% TG High Risk <40 mg% 37.6 mg% HDL-C Negative Risk > 60 mg% mg% 226.4 mg% LDL-C 8-32 mg% 36 mg% VLVL-C Up to 3.22 7.98 T. Chol/HDL-C Risk Ratio Up to 4.5 6.02 LDL-C/HDL-C Risk Ratio

26 Hormonal Profile Thyroid Hormones Sex Hormones Adrenal Hormones
Parathyroid Hormones Pituitary Hormones Pancreatic Hormones

27 Reference Rage Patient Result Test 2-4.4 pg/ml 1.94 pg/ml T3 free ng% 0.63 ng% T4 free μIU/ml 28.44 μIU/ml TSH

28 LH>FSH = polycystic ovary syndrome
LH:FSH ration should be 1:1

29 Reference Range Patient Result
Test Non pregnant <5mIU/ml 215 Β-HCG 0-1 Week 6-50 mIU/ml 1-2 Week mIU/ml 2-3 Week mIU/ml 3-4 Week mIU/ml 4-5 Week mIU/ml 5-6 Week mIU/ml 6-8 Week mIU/ml 2-3 Month mIU/ml 4-6 Month mIU/ml 7-9 Month mIU/ml

30 Tumor Markers

31 Tumor Markers Report Reference Rage Patient Result Test Up to 4 ng/ml
PSA, Total Up to 0.9 U/ml 2.8 U/ml PSA, Free Reference Rage Patient Result Test Up to 5 ng/ml 4.12 ng/ml CEA Up to 25 U/ml 292.7 U/ml CA 15-3

32 Diabetic Profile Fasting Blood Glucose (FBG)
Postprandial Blood Glucose Random Blood Glucose Fasting Insulin Glycated Hemoglobin (HA1c) HOMA-IR

33 Diabetic Profile Reference Rage Patient Result Test <6% 11% HbA1c
mg% 228 mg% FBG mg% 316mg% PBG

34 Parathyroid Hormone “PTH”
Osteoporosis Profile Total Calcium “Ca” Ionized Ca Phosphorus “Pi” ALP Parathyroid Hormone “PTH”

35 Reference Rage Patient Result Test 15-65 pg/ml 78.5 pg/ml PTH Deficiency <20 ng/ml 17.4 ng/ml Vitamin D total Insufficiency ng/ml Sufficiency >30 ng/ml

36 Immunological Profile
Serological & Immunological Profile Reference Rage Patient Result Test <200 IU/ml 600 IU/ml ASOT <6 mg/L 9 mg/L CRP <8 IU/L 12 IU/L RF Male: 0-10 mm/hr 40 mm/hr ESR

37 Gastrointestinal tract Thyroid autoantibodies Liver Systemic
Anti-tTG AGA Intrinsic Factor Antibodies Parietal Cell Antibodies Thyroid autoantibodies Anti-TPO TSH receptor antibodies Liver SMA AMA Anti-LKM1 Systemic ANA ANCA AntidsDNA ACA Antihistone CCP anti-SS-A (Ro) and anti-SS-B (La), anti-RNP, anti-Jo-1, anti-Sm, Scl-70 Organ-specific autoantibodies Cardiolipin Antibodies Beta-2 Glycoprotein 1 Antibodies APA LA Endocrine/metabolic system Diabetes-related Autoantibodies Kidney GMB Muscles AChR Examples of Autoantibodies Systemic autoantibodies The list below includes some of the autoantibody tests that are used to identify systemic autoimmune disorders. These disorders may cause signs and symptoms associated with inflammation throughout the body. Antinuclear Antibodies (ANA) Antineutrophil Cytoplasmic Antibodies (ANCA) Anti-Double Stranded DNA (anti-dsDNA) Anticentromere Antibodies (ACA) Antihistone Antibodies Cyclic Citrullinated Peptide Antibodies (CCP) Extractable Nuclear Antigen Antibodies (e.g., anti-SS-A (Ro) and anti-SS-B (La), anti-RNP, anti-Jo-1, anti-Sm, Scl-70) Rheumatoid Factor (RF) Organ-specific autoantibodies These are examples of autoantibodies associated with certain systems or organs: Clotting (coagulation) system Cardiolipin Antibodies Beta-2 Glycoprotein 1 Antibodies Antiphospholipid Antibodies (APA) Lupus anticoagulants (LA) Endocrine/metabolic system Diabetes-related Autoantibodies Gastrointestinal tract Anti-Tissue Transglutaminase (anti-tTG) Anti-Gliadin Antibodies (AGA) Intrinsic Factor Antibodies Parietal Cell Antibodies Thyroid Thyroid Autoantibodies (e.g., anti-TPO, TSH receptor antibodies) Liver Smooth Muscle Antibodies (SMA) Antimitochondrial Antibodies (AMA) Liver Kidney Microsome Type 1 Antibodies (anti-LKM-1) Kidney Anti-Glomerular Basement Membrane (GBM) Muscles Acetylcholine Receptor (AChR) Antibodies

38 Reference Rage Patient Result 60 IU/ml 30 U/ml
Test Negative <35 IU/ml 60 IU/ml Anti ds DNA Intermediate IU/ml Positive >45 IU/ml <10 U/ml 30 U/ml ANA +ve Anti ds DNA and ANA = systemic lupus

39 Laboratory Abbreviations See the attached Word file.

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