1. Horizon Chromatography
HPLC/UHPLC Columns

2. Introduction Horizon new HPLC, UHPLC and Core-Shell ranges
Ultra pure silica %
High surface area (300m2/g)
Excellent peak shapes
High resolution selectivity choices
We pride ourselves on our delivery and sales service
Technical Support service

3. Available Phases Aurashell – Core-Shell Aurashell C18
Horizon – Fully Porous (300m2/g)
Aurashell C18
Aurashell C18/PFP
Aurashell Aqua C18
Aurashell HILIC
Aurashell PEP C18 (160Å)
Aurashell PRO C4 (300Å)
Horizon C18
Horizon C18/PFP
Horizon Aqua C18
Horizon C8
Horizon HILIC
Horizon Phenyl
Horizon Amino
Horizon Cyano

4. Physicals Ultra pure 99.999% silica template
All phases are extensively endcapped
Horizon, porous particles
1.6µm UHPLC particles
3µm High efficiency particle
5µm standard ‘work horse’ particle
Aurashell, core-shell
2.7µm ultra high efficiency, low backpressure
5µm high efficiency, low backpressure

5. Physicals S.A. %C Pore Size pH range USP Purity Endcapped Horizon C18
300m2/g 20
120Å
1-11 L1
99.999%
Yes
Horizon Aqua 16
2-10
Horizon C18/PFP 15
Horizon C8 12 L7
Horizon Phenyl
2-8
L11
Horizon Cyano 7
1-6
L10
Horizon HILIC 4
L20
Horizon Amino 5 L8

6. Aurashell Aurashell Monodisperse core shell particle
High speed separations
Uniform packed bed, low backpressure
Multiple particle sizes
2.7µm
5µm
Reversed phase chemistries
Bioanalysis optimised phases

7. UHPLC 1.6µm Horizon particles for UHPLC
Fully porous ultra pure inert silica
Excellent peak shapes and reproducibility
High efficiency UHPLC results
Multiple orthogonal selectivity's available

8. Horizon C18 Horizon C18 HPLC and UHPLC particle sizes
Excellent performance general purpose C18 alkyl chain stationary phase with high stability
Excellent peak shapes
Excellent selectivity
Guaranteed batch to batch reproducibility

9. Horizon C18 Horizon C18 Ultra high purity silica 99.999%
300m2/g surface area
120Å pore size
pH range 1-11
USP : L1

10. Horizon C18 Column: Horizon C18 3µm 150x4.6mm
Mobile Phase: A: 10mM Ammonium Bicarbonate pH10
B: MeOH
Gradient : 60:40 – 100% B in 10minutes
Flow rate : 1ml/min
Wavelength: 220nm
Temperature: 30oC
Tramadol
Amitriptyline

11. Horizon C18 Column: Horizon C18 3µm 150x4.6mm
Mobile Phase: A: 10mM Ammonium formate pH3.5 B: MeCN
Gradient : 60:40 – 100% B in 10minutes
Flow rate : 1ml/min
Wavelength: 254nm
Temperature: 20oC
Rosuvastatin
Simvastatin
Atorvastatin

12. Horizon C18/PFP Horizon C18/PFP HPLC and UHPLC particle sizes
Alternative selectivity L1 columns
Offers strong hydrophobic, pi-pi and dipole interactions
Extend retention of halogenated compounds
Guaranteed batch to batch reproducibility

13. Horizon C18/PFP Horizon C18/PFP Ultra high purity silica 99.999%
300m2/g surface area
120Å pore size
pH range 2-10
USP : L1

14. Horizon C18/PFP 1,2,3 5,6 4 Traditional C18 7 8 3 4 5 Horizon C18/PFP
Column: 3µm 150x4.6mm
Mobile Phase: 50:50 Water: MeOH
Flow rate : 1ml/min
Wavelength: 254nm
Temperature: 40oC
1,2,3
5,6 4
Traditional C18 7 8 3 4
1,2,3-Trimethoxybenzene
1,2,4-Trimethoxybenzene
1,2-Dimethoxybenzene
1,4-Dimethoxybenzene
Methoxybenzene
1,3-Dimethoxybenzene
1,3,5-Trimethoxybenzene
Toluene 5
Horizon C18/PFP 6 2 7 8 1

15. Horizon C18/PFP Column: Horizon C18/PFP 3µm 150x4.6mm
Mobile Phase: A: 0.1% Formic acid
B: 0.1% Formic acid in MeCN
Gradient : 10 – 100% B in 10minutes
Flow rate : 1ml/min
Wavelength: 254nm
Temperature: 20oC
Paracetamol
Hydrochlorothiazide
Methylphenylsulfoxide
Methylphenylsulfone

16. Horizon AQUA C18 Horizon Aqua C18 HPLC and UHPLC particle sizes
Excellent performance C18 with capability to operate in 100% aqueous mobile phases
Resistant to retention loss caused by highly aqueous mobile phases
Analyse polar compounds

17. Horizon AQUA C18 Horizon Aqua C18 Ultra high purity silica 99.999%
300m2/g surface area
120Å pore size
pH range 2-10
USP : L1

18. Horizon AQUA C18 Column: Horizon Aqua C18 5µm 150x4.6mm
Mobile Phase: A: 50mM KH2PO4 pH 3.5
B: MeOH
Gradient : 0 – 100% B in 15minutes
Flow rate : 1ml/min
Wavelength: 205nm
Temperature: 30oC
Vitamin B1
Vitamin B6
Vitamin B3
Vitamin B5
Folic acid
Vitamin B12
Vitamin H
Vitamin B2

19. Horizon AQUA C18 Column: Horizon Aqua C18 5µm 150x4.6mm
Mobile Phase: A: 0.1% TFA
B: 0.1% TFA in MeOH
Gradient : 0 – 100% B in 15minutes
Flow rate : 1ml/min
Wavelength: 270nm
Temperature: 20oC
Dopamine
Serotonin
DOPAC

20. Peptide/Protein Columns
Horizon BIO phases
Horizon fully porous PEP C18 and PRO C4
PEP C18 = 200Å C18 designed for peptide analysis and mapping
PRO C4 = 300Å C4 for wide range of protein analysis
Aurashell PEP C18
2.7µm core shell
160Å pore size
High efficiency phase for peptides
Aurashell PRO C4
3.5µ, core shell
300Å pore size
High efficiency phase for proteins

21. Aurashell PEP C18 Aurashell PEP C18 Monodisperse 160Å pore size
C18 ligand (6% C)
pH range 1-9
USP : L1

22. Aurashell PEP C18

23. Aurashell PEP C18 Tryptic digest 2.7µm Aurashell PEP C18 150x2.1mm
60 minute gradient
30 minute gradient
20 minute gradient

24. Aurashell PRO C4 Aurashell PRO C4 Monodisperse 300Å pore size
C4 ligand (1% C)
pH range 1-9
USP : L26

25. Aurashell PRO C4

26. Aurashell PRO C4 Protein Mix 3.5µm Aurashell PRO C4 100x2.1mm
Ribonuclease A (13.7kDa)
Cytochrome C (12kDa)
Halo-Transferrin (77kDa)
Apomyoglobin (17kDa)

27. Other Dimensions Preparative Columns 10mm or 21.2mm diameters
Ultra High purity 5µm or 10µm particles
High surface area
High sample loading
Excellent sample recovery
Capillary 75µm i.d.
Available with Horizon or Aurashell particles
Full range of bonded phases
Standard and custom made lengths available

28. Guards and Filters Horizon Guards:
Significantly increase the lifetime of columns
Prevent contamination
Available in all phase chemistries
Horizon Analytical pre-column filter:
2µm porosity frit
400bar pressure rated
Finger tight
Horizon UHPLC pre-column filter:
0.5µm porosity frit
1000bar pressure rated
Compatible with all UHPLC systems

29. Conclusion Horizon phases offer: Selectivity Efficiency
Excellent peak shapes
UHPLC 1.6µm particles
Aurashell phases offer:
High Efficiency
High Resolution
High Speed

30. E-mail : info@horizonhplc.com
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