1. Copyright © 1998 American Medical Association. All rights reserved.
From: Reattachment Rate of Human Retinal Pigment Epithelium to Layers of Human Bruch's Membrane
Arch Ophthalmol. 1998;116(3): doi: /archopht
Figure Legend:
Reattachment rate of human retinal pigment epithelium (RPE) to different layers of Bruch's membrane 6 hours after plating. Results are expressed in 3 groups: treated or untreated basal lamina (BL), deeper layers of Bruch's membrane, and controls. Retinal pigment epithelium reattachment to its own BL is highest, and is not altered by treatment with heparinase (BL+HEP), but is decreased by treatment with chondroitinase (BL+CHOND) or heparinase and chondroitinase (BL+HEP+CHOND). The RPE reattachment rate decreases progressively as deeper layers of Bruch's membrane are exposed (untreated BL is greater than the inner collagenous layer [ICL], which is greater than the elastin layer [EL], which is greater than the outer collagenous layer [OCL]). Trypsin digestion (BL+trypsin) markedly decreased RPE reattachment, although washing with phosphate-buffered saline (PBS) did not. Attachment to tissue culture plastic (TCP) and agarose (no measurable attachment) served as positive and negative controls, respectively. Bar indicates SEM.
Date of download: 12/25/2017
Copyright © 1998 American Medical Association. All rights reserved.

2. Copyright © 1998 American Medical Association. All rights reserved.
From: Reattachment Rate of Human Retinal Pigment Epithelium to Layers of Human Bruch's Membrane
Arch Ophthalmol. 1998;116(3): doi: /archopht
Figure Legend:
Transmission electron microscopy of human Bruch's membrane explants. A, Retinal pigment epithelial basal lamina is visible after ammonium hydroxide treatment. B, Mechanical removal of the basal lamina exposes the inner collagenous layer. C, Treatment of (B) with collagenase exposes the elastin layer. D, Treatment of (C) with elastase exposes the outer collagenous layer. RPE-BL indicates retinal pigment epithelial basal lamina; ICL, inner collagenous layer; EL, elastin layer; OCL, outer collagenous layer; and CC-BM, basal lamina of the choriocapillaris.
Date of download: 12/25/2017
Copyright © 1998 American Medical Association. All rights reserved.

3. Copyright © 1998 American Medical Association. All rights reserved.
From: Reattachment Rate of Human Retinal Pigment Epithelium to Layers of Human Bruch's Membrane
Arch Ophthalmol. 1998;116(3): doi: /archopht
Figure Legend:
There was excellent correlation (r=0.93) between the optical density read on an enzyme-linked immunosorbent assay plate reader using a colorimetric assay and the number of viable retinal pigment epithelium (RPE) cells.
Date of download: 12/25/2017
Copyright © 1998 American Medical Association. All rights reserved.

4. Copyright © 1998 American Medical Association. All rights reserved.
From: Reattachment Rate of Human Retinal Pigment Epithelium to Layers of Human Bruch's Membrane
Arch Ophthalmol. 1998;116(3): doi: /archopht
Figure Legend:
Schematic describing preparation of different anatomical layers of Bruch's membrane. Explants containing native retinal pigment epithelium (RPE) on Bruch's membrane are harvested from human cadaver eyes. Treatment with ammonium hydroxide removes the RPE, but leaves the RPE basal lamina (RPE BL) intact. The basal lamina can be treated with heparinase and/or chondroitinase. The basal lamina can be removed with gentle suction, exposing the fibers of the inner collagenous layer (ICL). Treatment with collagenase removes the inner collagenous layer and exposes the native elastin layer (EL). Treatment with elastase removes the elastin layer and exposes the native outer collagenous layer (OCL). Choriocapillaris basal lamina (CC BL) is present in all preparations.
Date of download: 12/25/2017
Copyright © 1998 American Medical Association. All rights reserved.

5. Copyright © 1998 American Medical Association. All rights reserved.
From: Reattachment Rate of Human Retinal Pigment Epithelium to Layers of Human Bruch's Membrane
Arch Ophthalmol. 1998;116(3): doi: /archopht
Figure Legend:
Photograph of apical surface of the explant surrounded by a ring of agarose (AGR) prior to plating retinal pigment epithelium. Large choroidal vessels are seen deep to the apical surface of Bruch's membrane (BM).
Date of download: 12/25/2017
Copyright © 1998 American Medical Association. All rights reserved.

6. Copyright © 1998 American Medical Association. All rights reserved.
From: Reattachment Rate of Human Retinal Pigment Epithelium to Layers of Human Bruch's Membrane
Arch Ophthalmol. 1998;116(3): doi: /archopht
Figure Legend:
Reattachment of human retinal pigment epithelium (RPE) to different layers of Bruch's membrane as a function of age. Results are expressed in 3 groups: treated or untreated basal lamina, deeper layers of Bruch's membrane, and controls. Retinal pigment epithelium reattachment to basal lamina is similar for younger and older eyes. The RPE reattachment rate to the inner collagenous layer, elastin layer, or outer collagenous layer was consistently greater for Bruch's membrane explants harvested from younger eyes compared with those from older eyes. The reattachment rate to trypsin-digested explants was uniformly poor for both age groups; reattachment to explants washed 3 times in phosphate-buffered saline served as the positive control. BL indicates basal lamina; BL+HEP, basal lamina treated with heparinase; BL+CHOND, basal lamina treated with chondroitinase; BL+HEP+CHOND, basal lamina treated with heparinase and chondroitinase; ICL, inner collagenous layer; EL, elastin layer; OCL, outer collagenous layer; and PBS, phosphate-buffered saline. Bar indicates SEM.
Date of download: 12/25/2017
Copyright © 1998 American Medical Association. All rights reserved.

7. Copyright © 1998 American Medical Association. All rights reserved.
From: Reattachment Rate of Human Retinal Pigment Epithelium to Layers of Human Bruch's Membrane
Arch Ophthalmol. 1998;116(3): doi: /archopht
Figure Legend:
Schematic summary of the retinal pigment epithelial cell reattachment rate to each layer of Bruch's membrane.
Date of download: 12/25/2017
Copyright © 1998 American Medical Association. All rights reserved.