1. Biological catalysts
Enzymes

2. Day 1

3. Bell ringer on page
You are analyzing a compound in the laboratory. You find that it is made up of carbon, hydrogen, and oxygen in a ratio of two hydrogen atoms for each carbon atom. How will you classify the compound? A. lipid B. protein C. carbohydrate D. nucleic acid

5. Catalysts
A catalyst is a substance that speeds up the rate of a chemical reaction but is not itself changed by the reaction.

6. hydrogen peroxide water + oxygen 2H2O2 2H2O + O2
Hydrogen peroxide breaks down to water and oxygen
hydrogen peroxide
manganese oxide
water + oxygen
2H2O2
2H2O + O2
The escaping oxygen causes the foaming

7. Enzymes act as biological catalysts
They occur inside cells or are secreted by the cells.
Catalase is the enzyme that catalyses the break down of hydrogen peroxide.
Catalase

8. catalase amylase pepsin trypsin Enzymes are proteins
Proteins are long molecules that are folded into a specific shape.
catalase
amylase
pepsin
trypsin

9. The part of an enzyme where the reaction occurs is known as the active site

10. The molecule the enzyme acts on is known as the substrate molecule
Substrate molecules
Active site:
The site on the enzyme where the reaction occurs
Enzyme

11. The molecule the enzyme produces is known as the product molecule
Product molecules
Active site:
The site on the enzyme where the reaction occurs
Enzyme

12. The enzyme can be re-used

13. Each enzyme is specific to one substrate molecule or type of molecule
The lock and key hypothesis states that the active site specifically matches the shape of the substrate molecule
enzyme
active site

14. The rate of an enzyme controlled reaction is affected by temperature
At low temperatures enzyme controlled reactions go slowly because the molecules have low kinetic energy.

15. When temperature increases the reaction also increases as the molecules have more kinetic energy
But this only occurs up to the optimum temperature (usually about 40oC)
The temperature at which the rate of reaction is
fastest is known as the optimum temperature

16. After the optimum temperature the heat causes the enzyme to denature
The enzyme changes shape and the active site no longer matches the shape of the substrate molecule

17. Rates of enzyme reactions can be measured by recording the time for a substrate to disappear or a product appears
Controlled variables:
Volume and concentration of substrate (milk)
Volume and concentration of enzyme (trypsin)
pH (controlled by buffers)
Temperature
protein
polypeptides
trypsin
white
clear

18. Rate of reaction of an enzyme reaction changes at different temperatures
Optimum temperature
Enzyme
is denaturing
Molecules gain kinetic energy
Rate Of
Reaction
Temperature/oC

19. The activity and shape of enzymes is also affected by pH
Enzymes prefer to work at an optimum pH. Outside of its pH range the enzyme is denatured.
Optimum pH
pepsin
amylase
Rate Of
Reaction pH

20. When temperature increases the reaction also increases as the molecules have more kinetic energy
But this only occurs up to the optimum temperature (usually about 40oC)
The temperature at which the rate of reaction is
fastest is known as the optimum temperature

21. After the optimum temperature the heat causes the enzyme to denature
The enzyme changes shape and the active site no longer matches the shape of the substrate molecule

22. Enzymes are used in biological washing powders
Proteases break down the coloured, insoluble proteins that cause stains to smaller, colourless soluble polypeptides.
Can wash at lower temperatures

23. Enzymes are used in the food industry
Pectinase break down substances in apple cell walls and enable greater juice extraction.
Lactase breaks down lactose in milk into glucose and galactose. This makes milk drinkable for lactose intolerant people.

24. Enzymes are used in seed germination
starch
amylase
secreted
embryo plant
absorbed
maltose

25. Summary Keywords: catalyst catalyse protein catalase amylase lactase
pectinase
trypsin
pepsin
substrate
active site
product
temperature
optimum
denature pH
protease
enzyme

26. Let’s Graph!
Make sure you are completing the worksheet as we go through it in class

27. Exit Ticket Complete silently and independently
I will collect when everyone has finished
Please sit quietly when you are done until I collect the exit tickets

28. Homework Complete the lactose case study worksheet due next class
Study for the macromolecule and enzyme quiz on 9/8 (A day) and 9/11 (B day)

29. Day 2

30. Bell ringer on page
Read the short introduction to the lab and then answer the pre-lab questions. This should go on page in your notebook.

31. Liver Lab Follow all directions at the three stations carefully
You will be given 20 minutes per station, then rotate as a group
Do not play with or otherwise touch the liver mixture
This lab will be worth 100 points and will be graded for accuracy

32. Post Lab Analysis
You have a few minutes to review and work on the post lab questions
The lab will be due, completed, at the beginning of next class

33. Homework Study for the macromolecule and enzyme quiz next class
Complete the liver lab