1. Ovine secondary follicles vitrified out the ovarian tissue grow and develop in vitro better than those vitrified into the ovarian fragments 
Franciele Osmarini Lunardi, Francisco Leo Nascimento de Aguiar, Ana Beatriz Graça Duarte, Valdevane Rocha Araújo, Laritza Ferreira de Lima, Naiza Arcângela Ribeiro de Sá, Hudson Henrique Vieira Correia, Sheyla Farhayldes Souza Domingues, Cláudio Cabral Campello, Johan Smitz, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues 
Theriogenology 
Volume 85, Issue 7, Pages (April 2016)
DOI: /j.theriogenology
Copyright © 2016 Elsevier Inc. Terms and Conditions

2. Fig. 1
Percentage of isolated morphologically normal sheep preantral follicles without vitrification (control, n = 50) and after vitrification of isolated follicles (follicle-vit, n = 40) or ovarian tissue (tissue-vit, n = 64) at Days 0, 6, 12, and 18 of IVC. ABDifferent uppercase letters indicate statistically significant differences among groups (P < 0.05) within the same day. abDifferent lowercase letters indicate statistically significant differences among days of culture (P < 0.05) within the same group.
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

3. Fig. 2
Normal sheep secondary follicle before (Day 0) IVC (A) or cultured for 6 days (B), 12 days (C), or 18 days (D) in follicle-vit group. Note beginning of antrum formation at Day 6 of IVC. (E) Percentage of antrum formation of sheep preantral follicles without vitrification (control, n = 39) and after vitrification of isolated follicles follicle-vit, n = 39) or ovarian tissue (tissue-vit, n = 60) at Days 6, 12, and 18 of IVC. ABDifferent uppercase letters indicate statistically significant differences among groups (P < 0.05) within the same day. abDifferent lowercase letters indicate statistically significant differences among days of culture (P < 0.05) within the same group.
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

4. Fig. 3
Follicular diameter (μm; mean ± standard error of the mean) of sheep preantral follicles without vitrification (control, n = 21) and after vitrification of isolated follicles (follicle-vit, n = 38) or ovarian tissue (tissue-vit, n = 44) at Days 0, 6, 12, and 18 of IVC. AThe uppercase letter indicates statistically significant differences among groups (P < 0.05) within the same day. abcDifferent lowercase letters indicate statistically significant differences among days of culture (P < 0.05) within the same group.
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

5. Fig. 4
(A) Daily growth rate (μm/day; mean ± standard error of the mean) of sheep preantral follicles without vitrification (control, n = 21) and after vitrification of isolated follicles (follicle-vit, n = 38) or ovarian tissue (tissue-vit, n = 44) after 18 days of IVC and (B) Percentage of sheep preantral follicles with decreased diameter from control (n = 18), follicle-vit (n = 1), and tissue-vit (n = 16) after 18 days of culture. ABCDifferent uppercase letters indicate statistically significant differences among groups (P < 0.05).
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions

6. Fig. 5
Viable oocyte in metaphase I after IVM from follicle-vit group after IVC for 18 days. Brightfield (A), Calcein-AM (B), and Hoechst (C).
Theriogenology  , DOI: ( /j.theriogenology )
Copyright © 2016 Elsevier Inc. Terms and Conditions