1. Human isoforms of NADPH oxidase catalytic subunit

2. Redox signaling in physiology
Growth and maintenance of neural stem cells
To trigger chemotaxis and recruitment of leukocytes to damaged tissue
To regulate circadian rhythms in the absence of transcription or translation
Dickinson and Chang, Nat Chem Biol 2012

3. Introduction
Early Evidence of EGF-mediated H2O2 Production and Redox Regulation of EGFR Signaling
Effect of H2O2 on Signaling Networks Downstream of EGFR
Interplay of Reversible Tyrosine Phosphorylation and Redox-Dependent Signaling
Generation and Metabolism of H2O2 during EGFR Signaling
Regulation of Intrinsic EGFR Tyrosine Kinase Activity through Cysteine Oxidation
Future Perspectives 

4. Key events related to redox regulation of EGFR signaling
through cysteine oxidation
Truong and Carroll, Biochemistry 2012

5. EGFR signaling pathways
Truong and Carroll, Biochemistry 2012

6. Oxidative modification of cysteine residues
by hydrogen peroxide
Truong and Carroll, Biochemistry 2012

7. Redox regulation of peroxiredoxins (Prxs)
during EGFR signaling
Truong and Carroll, Biochemistry 2012

8. General strategy for detecting protein
cysteine sulfenylation (RSOH) in cells
Truong and Carroll, Biochemistry 2012

9. Covalent cysteine-based protein targeting strategies
Truong and Carroll, Biochemistry 2012

10. Model for H2O2-dependent regulation of EGFR tyrosine kinase activity
Truong and Carroll, Biochemistry 2012

11. Conclusions In this presentation, it was highlighted:
the emerging and rapidly expanding role of redox regulation
during EGFR signaling
(ii) the unique chemistry of reactive cysteine residues within specific
target proteins, including EGFR
(iii) the co-localization of the oxidant sources with the redox-regulated
target protein
the value of selective and cell-permeable chemical approaches to
elucidating regulatory mechanisms that govern H2O2-mediated
sulfenylation of proteins
Given that aberrant sulfenylation of proteins is linked to aggressive cancer
phenotypes, these discoveries may form the basis for the development of
new therapeutic anti-cancer strategies