2. Urine Analysis & Other Body Fluids (UBF) or Clinical Microscopy MLT 409
DR. BERNARD C. SILVALA

3. Clinical Microscopy or UBF
The course deals with the macroscopic, chemical and microscopic study of the different non-blood body fluids and their by products

4. Clinical Microscopy or UBF
urine, feces, sputum, gastric and duodenal contents, cerebrospinal fluid, synovial fluid, transudates, exudates, sweat, seminal fluid, vaginal fluid and amniotic fluid.

5. Clinical Microscopy or UBF
Physical characteristics
Chemical examinations
Microscopic analysis
Clinical significance and reference ranges
Methods:
Colorimetric, turbidimetric, gravimetric, automation, chromatography, etc.

6. Clinical Microscopy or UBF
Pre-requisites:
Microbiology
Physiology

7. Clinical Microscopy or UBF
QUALITY ASSURANCE AND SAFETY
Quality Assurance
Quality Control
Quality Assessment

8. Clinical Microscopy SAFETY IN THE LAB Laboratory Hazards
biological hazards
sharp hazards
chemical hazards
radioactive hazards
electrical hazards
fire/explosive hazards
physical hazards

9. Clinical Microscopy MICROSCOPY Types of Microscopy
Brightfield Microscope
Care and Use of the Microscope

10. Clinical Microscopy RENAL FUNCTION Renal Anatomy Renal Blood flow
Renal Physiology
Functions
Processes
Glomerular Filtration
Tubular Reabsorption
Tubular Secretion

11. Clinical Microscopy Urinalysis
Introduction (History, Urine formation, composition, volume, specimen collection, handling)
Physical examination of urine
Chemical examination of urine
Microscopic examination of urine

12. Clinical Microscopy Other methods of urinalysis
Non-conventional methods
Automation

13. Clinical Microscopy Renal and Renal Associated diseases Glomerular
Tubular
Interstitial
Vascular
Renal Failure
Renal lithiasis
Metabolic disorders

14. Clinical Microscopy Other Exams for Renal diseases Basic terms
Urinary sediment Quantification
Stone Analysis
Assessment of GFR
Concentration tests
Tubular Secretory fxn tests

15. Clinical Microscopy Other Body Fluids Seminal fluid & Vaginal fluid
Pregnancy Test and HCG Assay
CSF
Synovial fluid
Serous fluid
Amniotic fluid
Sweat
Bronchial washings & bronchoalveolar lavage
Gastric and duodenal content analysis
Fecal analysis

16. Clinical Microscopy or UBF
Brunzel, Nancy A. Fundamentals of Urine and Body Fluid Analysis. W.B. Saunders, Philadelphia
Henry, John Bernard et al. Clinical Diagnosis by Laboratory Methods. 20th Edition, W.B. Saunders, Philadelphia
Estridge, Barbara et al. Basic Medical Laboratory Techniques. 4th Edition. Delmar, USA
Leihmann, Craig A (ed). Saunder's Manual of Clinical Laboratory Science. W.B. Saunders, Philadelphia

17. Clinical Microscopy or UBF
McBride, Landy J. Textbook of Urinalysis and Body Fluids: A Clinical Approach. Lippincott, Philadelphia
Sacher, Ronald A & McPherson, Richard A. Widmann's Clinical Interpretation of Laboratory Tests. 11th Edition. F.A. Davis Company, Thailand
Strasinger, Susan K. & Di Lorenzo, Marjorie S. Urinalysis and Body Fluids. 4th Edition. F.A. Davis Company, Thailand