1. Therapeutic angiogenesis in diabetic apolipoprotein E-deficient mice using bone marrow cells, functional hemangioblasts and metabolic intervention 
Maria Luisa Balestrieri, Shi-Jiang Lu, Filomena de Nigris, Alfonso Giovane, Sharon Williams-Ignarro, Francesco Paolo D’Armiento, Qiang Feng, Carmela Fiorito, Gianluca Testa, Lucio Pastore, Francesco Cacciatore, Francesco Paolo Mancini, Luigi Servillo, Gaetano De Rosa, Caterina Pagliarulo, Monica Rienzo, Pellegrino Biagio Minucci, Bartolomeo Farzati, Francesco Salvatore, Franco Rengo, Louis Joseph Ignarro, Antonio Giordano, Andrew Baker, Robert Lanza, Claudio Napoli 
Atherosclerosis 
Volume 209, Issue 2, Pages (April 2010)
DOI: /j.atherosclerosis
Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

2. Fig. 1
Immunofluorescence analysis of vimentin, JNK-1, JNK-2, SIRT1, acetyl-FOXO1, FOXO1, p14ARF, and p16INK4A expression in HS and BMC. Cytospun of HS and BMC fixed with 3% paraformaldehyde and permeabilized with 0.1% Triton X-100 were incubated with specific antibodies against vimentin, JNK-1, or JNK-2, acetyl-FOXO1, SIRT1, p14ARF, and p16INK4A, and goat antibody against FOXO1. Secondary antibodies used were goat anti-mouse Alexa Fluor 488, goat anti-rabbit Alexa Fluor 633 and donkey anti-goat Alexa Fluor 488. Bar graph shows mean fluorescence intensity and colocalization for each antibody both for HS and BMC. Data are expressed as mean±SD and are representative of n=3 different experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Atherosclerosis  , DOI: ( /j.atherosclerosis )
Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

3. Fig. 2
3D images of HS and BMC. HS and BMC were fixed with 3% paraformaldehyde and permeabilized with 0.1% Triton X-100 before incubation with specific antibodies against vimentin, JNK-1, or JNK-2, acetyl-FOXO1, SIRT1, p14ARF, and p16INK4A, and goat antibody against FOXO1. Secondary antibodies were goat anti-mouse Alexa Fluor 488, goat anti-rabbit Alexa Fluor 633, and donkey anti-goat Alexa Fluor D images were reconstituted from each stack, deconvoluted using the 3D Blind Deconvolution algoritm of Autoquant software (Media Cybernetics Inc.) and the mean intensity of each probe was measured. (A and B) Bar graph shows relative fluorescence units (RFUs) for each antibody both for HS and BMC. Plots show the intensities of red and green channels together with the mean between the channels for the zy axis (right) and xz axis (left). (C) Bar graph shows the diameter and thickness of HS and BMC. Data are expressed as mean±SD and are representative of n=3 different experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Atherosclerosis  , DOI: ( /j.atherosclerosis )
Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

4. Fig. 2
3D images of HS and BMC. HS and BMC were fixed with 3% paraformaldehyde and permeabilized with 0.1% Triton X-100 before incubation with specific antibodies against vimentin, JNK-1, or JNK-2, acetyl-FOXO1, SIRT1, p14ARF, and p16INK4A, and goat antibody against FOXO1. Secondary antibodies were goat anti-mouse Alexa Fluor 488, goat anti-rabbit Alexa Fluor 633, and donkey anti-goat Alexa Fluor D images were reconstituted from each stack, deconvoluted using the 3D Blind Deconvolution algoritm of Autoquant software (Media Cybernetics Inc.) and the mean intensity of each probe was measured. (A and B) Bar graph shows relative fluorescence units (RFUs) for each antibody both for HS and BMC. Plots show the intensities of red and green channels together with the mean between the channels for the zy axis (right) and xz axis (left). (C) Bar graph shows the diameter and thickness of HS and BMC. Data are expressed as mean±SD and are representative of n=3 different experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Atherosclerosis  , DOI: ( /j.atherosclerosis )
Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

5. Fig. 3
Evaluation of blood flow and capillary density. Laser doppler-derived blood flow and capillary density analysis were performed as described under Section 2. Data are expressed as mean±SD of single measurement in each mouse of the study group. Ischemic hindlimb (IH), bone marrow cell (BMC), hemangioblasts (HS), metabolic treatment (MT). Inset: representative images of immunostaining of CD34+ cells in the ischemic muscle of diabetic mice. Brown spots are CD34+ cells. Blue represents cells counter-stained with hematoxylin. (A) treated with PBS saline infusion (IH), (B) BMC therapy alone (IH+BMC), (C) BMC therapy plus metabolic intervention (IH+BMC+MT), (D) metabolic intervention alone (IH+MT), (E) HS therapy alone (IH+HS), (F) HS therapy plus BMC (IH+HS+BMC), (G) IH with BMC and HS plus MT (IH+BMC+HS+MT), and (H) IH with HS plus MT (IH+HS+MT). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Atherosclerosis  , DOI: ( /j.atherosclerosis )
Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

6. Fig. 4
In vivo imaging of HS homing. Ischemic mice were treated either with HS or PBS. HS homing capacity was determined by in vivo imaging system (upper panel) and by immunohistochemistry evaluation of GFP and CD34 coexpression (lower panel). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Atherosclerosis  , DOI: ( /j.atherosclerosis )
Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions