1. GOOD MORNING

3. Dental caries

5. Drill n fill
Most common dental disease- DC…Even though the research is sky-rocketing…y r v ..DENTISTS… are being perceived as this???

6. What if…. There is a cost-effective method Additional health benefits
Probiotic combating streptococcus mutans

7. BUG
for a

8. Methods and materials

9. INCLUSION CRITERIA EXCLUSION CRITERIA Age: 20-25yrs
Medically fit
DMFS ≤ 6
Orthodontic treatment
Caries preventive therapy/ measures
Antibiotic therapy

10. 40
GROUP 1 PLACEBO (n=20)
GROUP 2
PROBIOTIC (n=20)
15 day study

11. 10 ml solution
Placebo – distilled water
Probiotic – 150 million Bacillus coagulans (Lactospore,SAMI,Bangalore,India) spores in distilled water

12. Saliva collection
Drink
1 hr

13. Consumption of solutions : swished for a minute and swallowed

14. (Wilcoxon , Mann Whitney U)
Saliva collection
Microbial analysis
Statistical analysis
(Wilcoxon , Mann Whitney U)

16. PLACEBO GROUP

17. PROBIOTIC GROUP

18. DAY 15 – PLACEBO N PROBIOTIC

19. Significant difference between
S mutans counts of Day 1 & Day 15 (p<0.05)
placebo and probiotic (p<0.001)

20. DISCUSSION

22. Probiotics
Live microorganisms, if administered in adequate amounts, confer a health benefit on the host
Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria, 1-4 October 2001

23. Krishnappa S, Srinath S, Bhardwaj P, CH Mallaya
Krishnappa S, Srinath S, Bhardwaj P, CH Mallaya. Role of Probiotics in Prevention of Dental Caries: A Review. J Adv Med Dent Scie 2013;1(2):86-91
G. Jindal et al; A comparative evaluation of probiotics on salivary mutans streptococci counts in Indian children; European archives of Paediatric Dentistry 12 (Issue 4). 2011
Anitha, et al. Probiotics for future caries control . Indian Journal of Dental Research, 24(5), 2013

24. Placebo – 10 ml of distilled water
Bacillus coagulans (Lactospore,SAMI,Bangalore,India) – 150 million spores in 10 ml of distilled water
Placebo – 10 ml of distilled water
Chinnappa A, Konde H, Konde S, Raj S, Beena JP. Probiotics for future caries control: A short-term clinical study. Indian J Dent Res 2013;24:547-9.
Krishnappa S, Srinath S, Bhardwaj P, CH Mallaya. Role of Probiotics in Prevention of Dental Caries: A Review. J Adv Med Dent Scie 2013;1(2):86-91

25. A cost-effective probiotic such as Bacillus coagulans might be a subject for further research for prevention of caries in children
Probiotic - probiotics have got immense potential in dental caries prevention
Bacterial interference with probiotic bacteria to support the stability and diversity of oral biofilms is gaining momentum in dentistry
G. Jindal et al; A comparative evaluation of probiotics on salivary mutans streptococci counts in Indian children; European archives of Paediatric Dentistry 12 (Issue 4). 2011
Role of Probiotics in Prevention of Dental Caries: A Review. Krishnappa S, Srinath S, Bhardwaj P, CH Mallaya. J Adv Med Dent Scie 2013;1(2):86-91
The use of probiotic products could be an alternative strategy of displacing pathogenic microorganisms by probiotic bacteria and can thus be exploited for the prevention of enamel demineralization
Are we ready for caries prevention through bacteriotherapy? Braz Oral Res., (São Paulo) 2012;26(Spec Iss 1):64-70
Anitha, et al. Probiotics for future caries control . Indian Journal of Dental Research, 24(5), 2013

27. Conclusion

28. Use of Bacillus coagulans-novel, cost-effective, user-friendly
One of a kind - clinical trial

29. Smart dentist

30. thanq