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Oil Red O positive vacuolated blasts in a case of CD45 negative, CD19 negative B- lymphoblastic leukemia  Diana O. Treaba, MD, Allison Chen, Chad Ellermeier,

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Presentation on theme: "Oil Red O positive vacuolated blasts in a case of CD45 negative, CD19 negative B- lymphoblastic leukemia  Diana O. Treaba, MD, Allison Chen, Chad Ellermeier,"— Presentation transcript:

1 Oil Red O positive vacuolated blasts in a case of CD45 negative, CD19 negative B- lymphoblastic leukemia  Diana O. Treaba, MD, Allison Chen, Chad Ellermeier, MD, Bradley DeNardo, MD, Rachel Altura, MD  Human Pathology: Case Reports  Volume 6, Pages 1-7 (December 2016) DOI: /j.ehpc Copyright © 2016 The Authors Terms and Conditions

2 Fig. 1 CT image of the anterior mediastinal mass.
Human Pathology: Case Reports 2016 6, 1-7DOI: ( /j.ehpc ) Copyright © 2016 The Authors Terms and Conditions

3 Fig. 2 Blast with intracytoplasmic vacuoles; peripheral blood smear, Wright–Giemsa stain, immersion oil, 1000×. (A) Numerous vacuolated blasts; bone marrow aspirate smear, Wright–Giemsa stain, immersion oil, 1000× (B). Human Pathology: Case Reports 2016 6, 1-7DOI: ( /j.ehpc ) Copyright © 2016 The Authors Terms and Conditions

4 Fig. 3 Blasts positive with Oil Red O; bone marrow aspirate, immersion oil, 1000×. (A) Anti-myeloperoxidase antibody; bone marrow biopsy, 500× (B). Human Pathology: Case Reports 2016 6, 1-7DOI: ( /j.ehpc ) Copyright © 2016 The Authors Terms and Conditions

5 Fig. 4 Flow cytometry immunophenotypic analysis of the bone marrow aspirate. The blasts are CD45 negative. Human Pathology: Case Reports 2016 6, 1-7DOI: ( /j.ehpc ) Copyright © 2016 The Authors Terms and Conditions

6 Fig. 5 Flow cytometry immunophenotypic analysis. The blasts are CD34 positive and CD19 negative (A), and are partially surface CD22 positive and CD20 negative (B). Human Pathology: Case Reports 2016 6, 1-7DOI: ( /j.ehpc ) Copyright © 2016 The Authors Terms and Conditions

7 Fig. 6 Bone marrow biopsy. The blasts are positive for PAX-5 (A) and TdT (B); 500× immersion oil. Human Pathology: Case Reports 2016 6, 1-7DOI: ( /j.ehpc ) Copyright © 2016 The Authors Terms and Conditions

8 Fig. 7 Bone marrow biopsy. The blasts are variably positive for CD43 (A) and less than 5% of the blasts are positive for CD79a (B); 500× immersion oil. Human Pathology: Case Reports 2016 6, 1-7DOI: ( /j.ehpc ) Copyright © 2016 The Authors Terms and Conditions

9 Fig. 8 Karyotype, bone marrow aspirate, of the current B lymphoblastic leukemia case. Human Pathology: Case Reports 2016 6, 1-7DOI: ( /j.ehpc ) Copyright © 2016 The Authors Terms and Conditions


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