1. Properties of Water Polar molecule Cohesion and adhesion
High specific heat
Density – greatest at 4oC
Universal solvent of life

2. Polarity of Water
In a water molecule two hydrogen atoms form single polar covalent bonds with an oxygen atom. Gives water more structure than other liquids
Because oxygen is more electronegative, the region around oxygen has a partial negative charge.
The region near the two hydrogen atoms has a partial positive charge.
A water molecule is a polar molecule with opposite ends of the molecule with opposite charges.
2 corners are orbitals with unshared electrons and a weak negative charge
2 are occupied by hydrogen atoms that have polar covalent bonds

3. Water has a variety of unusual properties because of attractions between these polar molecules.
The slightly negative regions of one molecule are attracted to the slightly positive regions of nearby molecules, forming a hydrogen bond.
Each water molecule can form hydrogen bonds with up to four neighbors.
Fig. 3.1
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4. HYDROGEN BONDS
Extraordinary Properties that are a result of hydrogen bonds.
Cohesive behavior
Resists changes in temperature
High heat of vaporization
Expands when it freezes
Versatile solvent
Hold water molecules together
Each water molecule can form a maximum of 4 hydrogen bonds
The hydrogen bonds joining water molecules are weak, about 1/20th as strong as covalent bonds.
They form, break, and reform with great frequency

5. Organisms Depend on Cohesion
Hydrogen bonds hold the substance together, a phenomenon called cohesion
Cohesion is responsible for the transport of the water column in plants
Cohesion among water molecules plays a key role in the transport of water against gravity in plants
Adhesion, clinging of one substance to another, contributes too, as water adheres to the wall of the vessels.

6. Surface tension, a measure of the force necessary to stretch or break the surface of a liquid, is related to cohesion.
Water has a greater surface tension than most other liquids because hydrogen bonds among surface water molecules resist stretching or breaking the surface.
Water behaves as if covered by an invisible film.
Some animals can stand, walk, or run on water without breaking the surface.
Fig. 3.3
Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings

7. Specific Heat is the amount of heat that must be absorbed or lost for one gram of a substance to change its temperature by 1oC.
Three-fourths of the earth is covered by water. The water serves as a large heat sink responsible for:
Prevention of temperature fluctuations that are outside the range suitable for life.
Coastal areas having a mild climate
A stable marine environment

8. Evaporative Cooling
The cooling of a surface occurs when the liquid evaporates
This is responsible for:
Moderating earth’s climate
Stabilizes temperature in aquatic ecosystems
Preventing organisms from overheating

9. Density of Water Most dense at 4oC Contracts until 4oC
Expands from 4oC to 0oC
The density of water:
Prevents water from freezing from the bottom up.
Ice forms on the surface first—the freezing of the water releases heat to the water below creating insulation.
Makes transition between season less abrupt.

10. Ice is about 10% less dense than water at 4oC.
When water reaches 0oC, water becomes locked into a crystalline lattice with each molecule bonded to to the maximum of four partners.
As ice starts to melt, some of the hydrogen bonds break and some water molecules can slip closer together than they can while in the ice state.
Ice is about 10% less dense than water at 4oC.
Fig. 3.5
Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings

11. Solvent for Life Solution Aqueous solution Hydrophilic Hydrophobic
Solute
solvent
Aqueous solution
Hydrophilic
Ionic compounds dissolve in water
Polar molecules (generally) are water soluble
Hydrophobic
Nonpolar compounds

12. Most biochemical reactions involve solutes dissolved in water.
There are two important quantitative proprieties of aqueous solutions.
1. Concentration
2. pH

13. Concentration of a Solution
Molecular weight – sum of the weights of all atoms in a molecule (daltons)
Mole – amount of a substance that has a mass in grams numerically equivalent to its molecular weight in daltons.
Avogadro’s number – 6.02 X 1023
A mole of one substance has the same number of molecules as a mole of any other substance.

14. Dissociation of Water Molecules
Occasionally, a hydrogen atom shared by two water molecules shifts from one molecule to the other.
The hydrogen atom leaves its electron behind and is transferred as a single proton - a hydrogen ion (H+).
The water molecule that lost a proton is now a hydroxide ion (OH-).
The water molecule with the extra proton is a hydronium ion (H3O+).
Unnumbered Fig. 3.47
Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings

15. This reaction is reversible.
A simpler way to view this process is that a water molecule dissociates into a hydrogen ion and a hydroxide ion:
H2O <=> H+ + OH-
This reaction is reversible.
At equilibrium the concentration of water molecules greatly exceeds that of H+ and OH-.
In pure water only one water molecule in every 554 million is dissociated.
At equilibrium, the concentration of H+ or OH- is 10-7M (25°C) .
Only a hydrogen ion is actually transferred
The transferred proton binds to an unshared orbital of the second water molecule.
The water molecule that loses the proton

16. Acids and Bases
An acid is a substance that increases the hydrogen ion concentration in a solution.
Any substance that reduces the hydrogen ion concentration in a solution is a base.
Some bases reduce H+ directly by accepting hydrogen ions.
Strong acids and bases complete dissociate in water.
Weak acids and bases dissociate only partially and reversibly.
There is only 1 molecule out of 554,000,000 water molecules that is dissociated.

17. pH Scale The pH scale in any aqueous solution :
[ H+ ] [OH-] = 10-14
Measures the degree of acidity (0 – 14)
Most biologic fluids are in the pH range from 6 – 8
Each pH unit represents a tenfold difference (scale is logarithmic)
A small change in pH actually indicates a substantial change in H+ and OH- concentrations.

18. Problem
How much greater is the [ H+ ] in a solution with pH 2 than in a solution with pH 6?
Answer:
pH of 2 = [ H+ ] of 1.0 x 10-2 = 1/100 M
pH of 6 = [ H+ ] of 1.0 x 10-6 = 1/1,000,000 M
10,000 times greater

19. Buffers A substance that eliminates large sudden changes in pH.
Buffers help organisms maintain the pH of body fluids within the narrow range necessary for life.
Are combinations of H+ acceptors and donors forms in a solution of weak acids or bases
Work by accepting H+ from solutions when they are in excess and by donating H+ when they have been depleted.