1. Marina Treneva1, Daniel Munblit 2, Alexander Pampura1
MOSCOW (RUSSIA) WOMEN WITH POSITIVE SKIN PRICK-TESTS: COLOSTRUM AND BREAST MILK CYTOKINES AS AN ADOPTIVE PROTECTIVE MECHANISM FOR THE OFFSPRING
Imperial College London
Pirogov Russian National Research Medical University
Marina Treneva1, Daniel Munblit 2, Alexander Pampura1
1 Research and Clinical Institute for Pediatrics at
the Pirogov Russian National Research Medical University, Moscow, Russia
 2 Imperial College London, London, United Kingdom
Research and Clinical Institute for Pediatrics (Moscow)
Methods:
277 Moscow women were recruited as a part of an International (Great Britain, Russia, Italy) study assessing immune composition of colostrum and breast milk.
19,4% of Moscow women were sensitized to one or more allergens (D.pteronissinus, Cat, Birch pollen, Grass pollen, Ryegrass, Hazelnut, Peanut, Egg, Cow milk).
Electrochemiluminiscence of colostrum (204 samples) and breast milk (153 samples) for HGF,
IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, TGF-β-1, TGF-β-2, TGF-β-3 was performed
in a Flow Cytometry Lab, Imperial College
Background: There is some evidence to suggest that colostrums/breast milk immunological composition have potential in infant gut immunity modulation early in life
Results: According to univariate analysis colostrum and/or breast milk levels of TGF-β-1, TGF-β-3, HGF, IL-5, IL-10 and IFN-γ were higher in women with positive skin prick-tests.
No difference in IL-2, IL-4, IL-12, IL-13, TGF-β-2 levels was revealed in relation to positive skin prick tests.
Table 1 contains results of all examples analysis without adjustment.
Table 1
Reliable distinctions in colostrum and breast milk cytokine composition in Russian women depending on skin prick-test papule diameter
Objective:  to assess levels of cytokines in colostrum and breast milk in women with sensitization.
Absolute values
Lg of absolute values
colostrum
breast milk
Papule ≥ 1 mm to one or more allergens
no difference in
HGF, IFN-γ, IL-2,
IL-4, IL-5, IL-10, IL-12, IL-13, TGF-β-2
no difference in IL-2, IL-4, IL-10, IL-12, IL-13,
TGF-β-1, TGF-β-2, TGF-β-3;
reliable distinctions in
HGF p=0,037;
IFN-γ p=0,042
no difference in HGF, IFN-γ, IL-2, IL-4, IL-5, IL-10,
IL-12, IL-13, TGF-β-2;
reliable distinctions in TGF-β-1 р=0,029
no difference in IL-4, IL-5,
IL-10, IL-12, IL-13,
reliable distinctions in HGF p=0,020; IFN-γ p=0,002
Papule ≥ 3 mm to one or more allergens
no difference in HGF, IFN-γ, IL-2, IL-4,
IL-5, IL-10, IL-12, IL-13, TGF-β-2
no difference in IFN-γ,
IL-2, IL-4, IL-10, IL-12,
IL-13, TGF-β-1, TGF-β-2, TGF-β-3;
HGF p=0,013
no difference in HGF, IFN-γ, IL-2, IL-5, IL-10, IL-12,
IL-13, TGF-β-2;
reliable distinctions in TGF-β-1 р=0,003;
TGF-β-3 р=0,040
no difference in IL-4, IL-5, IL-10, IL-12,
IL-13, TGF-β-1,
TGF-β-2, TGF-β-3;
reliable distinctions in HGF p=0,012; IFN-γ p=0,005
Conclusion: colostrum and breast milk cytokine and growth factors in Moscow women allow to assume the lack of IL-4 and IL-13 shift in relation to sensitization.
There may be an assumption that the increased levels of growth factors, IL-5, IL-10 and IFN-γ revealed could be the adoptive mechanisms in sensitized women to protect their offspring from an allergy, according to the unique regenerative nature of a human body.
This hypothesis should be tested further to exclude influence of maternal and environment factors on results of the analysis, in connection with heterogeneity of the analyzed groups.