1. From: An In Vivo Doxycycline-Controlled Expression System for Functional Studies of the Retina
Invest. Ophthalmol. Vis. Sci ;44(2): doi: /iovs
Figure Legend:
(A) Rho-tTA1 construct used to generate transgenic mice. The 4.4-kb genomic region of the rhodopsin gene, 5′ to the start codon, was cloned upstream to tTA1 in the pUHD21-1 vector using KpnI and XbaI restriction sites. The construct was engineered to replace the rhodopsin start codon with tTA1 ATG. (B) Southern blot analysis of the two transgenic lines. Identical amounts of genomic DNA (12 μg) were loaded in the two lanes. Arrow: size of the transgene unit. (C) RT-PCR on cDNA from mouse eyes at different ages after birth (P6, P7, P8, P9, P13, and adult). (−) RT-PCR in the absence of reverse transcriptase.
Date of download: 12/29/2017
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