1. Pkc#cod#x003B4; Activation is Involved in ROS-Mediated Mitochondrial Dysfunction and Apoptosis in Cardiomyocytes Exposed to Advanced Glycation End Products (Ages)
Yang Yao-Chih 1 ;Tsai Cheng-Yen 2, 3 ;Chen Chien-Lin 4 ;Kuo Chia-Hua 5, 6 ;Hou Chien-Wen 5 ;Cheng Shi-Yann 7, 8, 9 ;Aneja Ritu 10 ;Huang Chih-Yang 11 ;Kuo Wei-Wen 1 ;
1 Department of Biological Science and Technology, College of Biopharmaceutical and Food Sciences, China Medical University, Taiwan. 2 Department of Pediatrics, China Medical University Beigang Hospital, Taiwan. 3 School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taiwan. 4 Department of Life Sciences, National Chung Hsing University, Taiwan. 5 Laboratory of Exercise Biochemistry, University of Taipei, Taipei, Taiwan. 6 Graduate Institute of Physical Therapy and Rehabilitation Science, China Medical University, Taiwan. 7 Department of Medical Education and Research and Department of Obstetrics and Gynecology, China Medical University Beigang Hospital, Taiwan. 8 Department of Obstetrics and Gynecology, China Medical University An Nan Hospital, Taiwan. 9 Obstetrics and Gynecology, School of Medicine, China Medical University, Taichung, Taiwan. 10 Department of Biology, Georgia State University, Atlanta, GA 30303, USA. 11 Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan ; Graduate Institute of Chinese Medical Science, School of Chinese Medicine, China Medical University, Taichung, Taiwan ; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan. ;
Figure 3. Cardiac PKC#cod#x003B4; expression and phosphorylation as well as apoptosis-related proteins are increased in diseased animal models with elevated circulatory AGE levels. Western blot analysis of the cardiac expression and phosphorylation levels of PKC isoforms and apoptosis-related proteins in rats with A diabetes mellitus DM and B a high-fat HF diet. Serum AGE levels in rats with C DM; D HF diet. These are cropped blots; full-length blots are presented in Suppl. Figure S3. Protocols for animal models with DM and HF diets and serum AGE analysis were described in the methods section. E Cardiac expression of phosphorylated PKC#cod#x003B4; was examined by immunohistochemistry analysis. Bars indicate the mean #cod#x000B1; SEM obtained from experiments performed in triplicate. #cod#x0002A; P #cod#x0003C;0.05 and #cod#x0002A;#cod#x0002A;#cod#x0002A; P #cod#x0003C;0.001 compared with the control group. DM, Diabetes mellitus; HF, high-fat diet.
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