1. Micropropagation of Uraria picta (Jacq.) DC
Mafatlal M .Kher and M. Nataraj
B.R.D. School of Biosciences,
Sardar Patel University,
Vallabh Vidyanagar,
Gujarat,
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Introduction
Belongs to Fabaceae
Common name Pithwan or Prishnaparni
Distributed throughout India, China, Burma, Shrilanka (Yadav et al., 2009)
Traditional uses
Antidote to snake venom (Kirtikar and Basu, 1993)
Cough and fever (Kirtikar and Basu,1993; Yusuf et al., 1994)
Other property
Antimicrobial ( Rahman et al2007)
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Pharmaceutical products
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As a result of this endangerment, in India the plant has been categorized as rare or vulnerable in the natural environment, making conservation of this plant crucial.
Plant tissue culture in such cases has been shown to be useful method which allows mass multiplication under aseptic conditions and it is not dependent on season for availability of plant material.
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In the present research work using this technique, the efforts were made to made to develop a method of rapid multiplication, by studying potential of coteledenory node as explant for micropropagation study.
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Materials and Methods
Media Preparation
Plant Growth Regulators
Surface sterilization
Seed Germination
Axillary shoot multiplication
Subculture
Rooting
Culture condition
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Seed germination
Effect of Different concentrations of GA3 on seed germination of Uraria picta
Six seeds per vessel with four replicates for each concentration
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8. Shoot multiplication and subculture
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9. Shoot multiplication and subculture
Effect of Different concentrations of Kinetin and BAP on axillary shoot proliferation from coteledenory node of Uraria picta Four explant per vessel with six replicates for each concentration
Effect of subculture on shoot multiplication each experiment with six replicate and four explant per culture bottle
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Rooting
Effect of different concentrations of NAA on in vitro rooting of Uraria picta each experiment with six replicate and four explant per culture bottle
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Thank you all.
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