1. Fibroblasts microinjected with RhoGEF expression constructs showing the promotion of actin polymerization through the activation of different Rho proteins. FGD1, Dbl, Lbc, and Vav promote the polymerization of actin in quiescent cells; serum-starved Swiss 3T3 cells were microinjected with plasmid DNA encoding epitope-tagged RhoGEF proteins.39 FGD1 stimulates the formation of filopodia (activation of Cdc42). In contrast, Dbl and Vav stimulate the formation of lamellipodia (activation of Rac), and Lbc stimulates the formation of actin stress fibers (activation of Rho). A construct containing an alternatively spliced form of FGD1 that lacks 36 amino acids near the N-terminal end of the GEF domain, FGD1Δ, fails to stimulate filopodial formation. Actin cytoskeletal structures in cells expressing the protein constructs were visualized with TRITC-conjugated phalloidin. Scale bar in A represents 20 μm and refers to all panels. (From Olsen et al.39 Used with permission of Current Biology.)
Source: Aarskog-Scott Syndrome, The Online Metabolic and Molecular Bases of Inherited Disease
Citation: Valle D, Beaudet AL, Vogelstein B, Kinzler KW, Antonarakis SE, Ballabio A, Gibson K, Mitchell G. The Online Metabolic and Molecular Bases of Inherited Disease; 2014 Available at: Accessed: January 01, 2018
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