1. Pseudomonas Identification
Done By :
Omniya Tayem
Dr: Adbelraouf Elmanama
Mohamad Laqan

2. Structure & Physiology
Pseudomonas
* Gram _ Negative rods
Gram negative rod

3. Motile with polar flagella *

4. Motility positive

5. Obligate aerobe

6. Oxidase _ positive *

7. Do not ferment Carbohydrates

8. Resistant to multiple drugs

9. Majority of human infections
_ P. aeruginosa

11. Common in the environment
. Water
. Air
.Soil

12. Very simple nutritional requirements
Many organic compound used as
C & N sources , but only
afew carbohydrates by
oxidative metabolism

13. Some strains appear mucoid due to
Polysaccharide Capsule

14. Some produce diffusible pigments
Pyocyanin _
nonfluorescent bluish pigment
Pyoverdin_
Fluorescent greenish pigment
Pyorubin & Pyomelanin

15. Fluorescent Pigments
White Light source
Black Light source

16. Pseudomonas . aeruginosa

17. Pseudomonas aeruginosa colonies on Agar

18. Pseudomonas . aeruginosa

19. Identification of P. Aeruginosa
Usually based on
colonial morphology ,
B- hemolysis ,
Oxidase positivity ,
The presence of characteristic pigments
& Sweet odor
& Growth at 42c

20. Identification Of Pseudomonas
Pigments *
Pyocyanin (blue _ green )
Fluorescein (green _yellow_fluorescent )
Pyorubin (red _ brown )

21. Culures have fruity smell *

22. Biochemical reactions *
.Gelatine hydrolysis
.Starch hydrolysis
.Carbohydrates use
Trehalose (O_F medium)
.Flourescent Pigment

23. Results

24. P. aeruginosa Gelatin hydrolysis +/ - ( variable result )
Starch hydrolysis + / - ( variable result )
Acid Aerobically from D_ Trehalose
( negative )
Fluorescent pigment ( positive )
Non Fluorescent pigment ( positive )

25. P .fluorescens Gelatin hydrolysis ( positive )
Starch hydrolysis ( negative )
Acid aerobically from D _ Trehalose (positive)
Fluorescent pigment ( negative )
Non fluorescent pigment (positive )

26. P. Saccharophila Gelatin hydrolysis (P) Starch hydrolysis (P)
Acid aerobically from D_ Trehalose (P )
Fluorescent pigment ( N )
Non fluorescent pigment ( N )

27. P. Putida Gelatine hydrolysis ( negative )
Starch hydrolysis (negative )
Acid aerobically from D_ Trehalose (N )
Fluorescent pigment ( P )
Non fluorescent pigment ( P )

28. The End