1. Rhizobium In Trigonella Root Nodule – A Natural Growth Enhancer – Studies its Effect on Sorghum vulgare & Cajanus cajan.
Aparna Gund, Sachin Bhagat, Dr. Umesh Kakde and Dr.Anil Bhalerao.
S.S. & L.S. Patkar College, Goregaon (west), Mumbai

2. INTRODUCTION
The most important cause of pollution is kitchen waste which can be used as bio fertilizer .
Which mostly ignore & thrown on dumping ground.
This causes pollution of precious land & spread disease such pollution can be avoided if treated at source that is kitchen garden.
The kitchen waste is created by cutting the vegetables, washing the vegetables, throwing the peels, roots, leaves or unrequired part of vegetables, which is rich in nutrition.
As the remaining roots of Trigonella foenum – graecum thrown into dustbin which is otherwise rich in root nodules containing rhizobium bacteria.
Trigonella foenum- graecum belongs to family- Fabaceae (Papilionaceae)

3. The kitchen waste like Trigonella roots can be treated as biofertiliizers for cultivation of commonly grown plants that is Sorghum & Cajanus.
We have taken this plants as this plants belongs to dicot & monocot to observe which gives better results.

4. Rhizobium is a gram negative, aerobic, rod-shaped bacterium
Rhizobium is a gram negative, aerobic, rod-shaped bacterium. It contains a refractive granule. It is a soil bacterium present in large numbers in rhizosphere of legume roots. Rhizobium invades roots of legumes and forms nodules on the roots.
These bacteria fix the atmospheric nitrogen into ammonia.
They provide the fixed nitrogen for plant’s use and draw nourishments from the root cells.
Different species of Rhizobium can fix kg nitrogen/ha/ year in leguminous crops. Therefore, they have been recommended as nitrogen bio fertilizers in agriculture.
Most of the Indian soils are deficient in nitrogen.
Hence require nitrogen fixing bacteria.
The bacteria helps in nitrogen fixation & growth.
Bio fertilizers are non-pollutant fertilizer.

5. Trigonella foenum-graecum and Sorghum vulgare
MATERIALS AND METHODS
Plant selection: - One dicotyledon and monocotyledon plants was selected.
Trigonella foenum-graecum and Sorghum vulgare
Methods for Preparation of Bio fertilizer stock solution:-
Trigonella plants were collected from the market.
Roots are cut and washed in water.
It is again washed with dilute alcohol to remove the soil particles.
100 gm. of roots is crushed with 100 ml water and made final volume 200 ml.
It is then diluted to make working solution of

6. Contrrol (0 ml stock solution + 100 ml water)
The working solutions were used for spraing instead of water.
The selection of pot :-
Small plastic pots of 1 kg capacity of soil.
The soil taken was a common garden soil and diluents were stored properly. Cultivation of plant :-
Plants were cultivated in different pots in triplicate for 7, 15, and 30 days
5 seeds were sown to avoid overcrowding.
The pots were kept in open place to get proper air and sunlight.
After 7, 15 and 30 days of germination various tests were conducted in two generations one in summer and in winter season.

7. RESULTS AND DISCUSSION
Entophytic Bacterial Colonies of Cajanus cajan.
25% concentration
50% concentration
Control
75% concentration
100% concentration

8. Photos of Sorghum vulgare 30 days plant of summer season.
50% concentration
Control
25% concentration
75% concentration
100% concentration

9. Morphological Analysis of Sorghum Vulgare Plant 30 Days of
Summer Season
Best result for Shoot Length were observed in 100% concentration.
Best result for No. of Leaves were observed in 100% concentration.
Best result for Root Length were observed in 100% concentration.

10. Photo of Cajanus Cajan 30 Days Plants of Summer Season.
Control
25 % concentration
50% concentration
75% concentration
100% concentration

11. Morphological Analysis of Cajanus cajan Plant 30 Days of Summer Season.
Best result for Shoot Length were observed in 25% concentration.
Best result for No of Leaves were observed in 75% concentration.
Best result for Root Length were observed in 75% concentration

12. Photos of 30 days Sorghum vulgare plant of winter season.

13. Morphological Analysis of Sorghum vulgare Plant 30 Days of Winter Season.
Best Result for Shoot Length were observed in 100% concentration
Best Result for No. of Leaves were observed in 100% concentration
Best Result for Root Length were observed in 100% concentration

14. Photo of Cajanus cajan 30 Days Plants of winter season.

15. Best Result for No. of Leaves Were observed in 75% concentration
Morphological Analysis of Sorghum vulgare Plant 30 Days of Winter Season.
Best Result for Shoot Length Were observed in 100% concentration
Best Result for No. of Leaves Were observed in 75% concentration
Best Result for Root Length Were observed in 50% concentration

16. From the above research work it was successfully demonstrate the effect of Trigonella foeinum gracum on Sorghum vulrare and Cajanus cajan. In Sorghum vulgare and Cajanus cajan of 30 days plants of summer season treated by 75% and 100% concentration of Trigonella extract showed good result for shoot length, root length and no. of leaves per plant than control.
In winter season Sorghum vulgare and Cajanus cajan of 30 days pants treated by 50%, 75% and 100% concentration shows good results.
From these results, we conclude that waste roots of Trigonella are a good resource of fertilizers. It is very important material for organic farming. Although lot of research is required to make better fertilizers which could support many more plants. When a large number of plants would be supporting such type of bio fertilizer, organic farming would reach to the highest level which would help the environment. The roots of Trigonella are the best resource material which could give accelerated and quantitative growth of Sorghum vulgare and Cajanus cajan.