PENGENALAN KULTUR MEDIA BAKTERI

PENGENALAN KULTUR MEDIA BAKTERI
DI EDIT OLEH PROF. DR.DRH .PRATIWI TS,MS 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

KULTUR MEDIA BACTERI UNTUK APA ?
9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

KULTUR MEDIA BAKTERI-PTS PET
APA YANG DIPERLUKAN? 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

KEBUTUHAN OKSIGEN 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

TUJUAN MELAKUKAN PENANAMAN

ISOLASI KULTUR MURNI 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

TIPE MEDIA TANAM 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

MEDIA CAIR 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

Kuliah berikut 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

fuchsine Gram Staining Technique

Selective Media Goal: To chemically (or physically) suppress unwanted microbes and encourage desired microbes. MSA Mannitol salt agar : selective for halophiles with 7% salt (osmotic challenge) and differential for mannitol fermenters: good for skin bacterial cultures. EMB Agar: kills gram positives with eosin and methylene blue, selective for gram negatives. Differential for lactose fermenters. Good for growing enterics. McConkey Agar: supresses gram positives with crystal violet and bile salts; also differential for EMB MA 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET Figure 6.9b, c

Differential Media Distinguish between different species based on a metabolic ability. Blood agar (sheep’s blood) reveals if hemolytic Mannitol salt agar contains the pH sensitive dye phenol red (yellow when acidic) Se Sa 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET Figure 6.9a

Direct Measurements of Microbial Growth
9/13/2018 KULTUR MEDIA BAKTERI-PTS PET Figure 6.19

Estimating Bacterial Numbers by Indirect methods
Direct Measures Plate counts of viable bacterial forming colonies Counting low viable bacterial numbers by filtration Counting viable bacteria with Most Probable Number Counting bacteria per ml in direct microscopy Indirect Measures Turbidity/Absorbance with a spectrophotometer Metabolic activity tracking conversion of colored molecules/enyzme assay Dry weight by weighing a set volume and knowing weight of one cell 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

Phases of Growth Lag Log Stationary Death
Adapt to nutrients Log Active growth Stationary Death = Growth rate Death Nutrients consumed pH too low (why?) Optimize curves in production 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

Log Growth 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

Growth Curve for Bacteria (Logarithmic Plot) 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET Figure 6.14

Estimating Bacterial Numbers by Indirect methods
Direct Measures Plate counts of viable bacterial forming colonies Counting low viable bacterial numbers by filtration Counting viable bacteria with Most Probable Number Counting bacteria per ml in direct microscopy Indirect Measures Turbidity/Absorbance with a spectrophotometer Metabolic activity tracking conversion of colored molecules Dry weight by weighing a set volume and knowing weight of one cell 9/13/2018 KULTUR MEDIA BAKTERI-PTS PET

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