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Supplemental Fig. 1 DLD1 DKO1 Log2 ratio DKO3 Chromosome
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Hec1A Hec1Aneo Log2 ratio Hec1Adelmut59 Chromosome
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Hec1Adelmut124 Hec1AdelWT34 Log2 ratio Hec1AdelWT277 Chromosome
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Supplemental Fig. 2 P-Akt P-ERK P-EGFR NRasGTP b-actin 10% FBS 0% FBS
0%FBS+EGF B A Mut/WT Mut/WT Mut/WT Mut/- -/WT Mut/- -/WT Mut/- -/WT P-Akt Mean fluorescent intensity P-ERK P-EGFR NRasGTP b-actin 0%FBS +EGF C 10% FBS 0% FBS 0%FBS+EGF D 10% FBS 0%FBS G12D/WT G12D/WT G12D/WT G12D/- WT/G13D WT/G13D WT/G13D G12D/- G12D/- -/WT -/WT WT/WT WT/WT WT/WT -/WT pY1125 pY1125 pY1069 pY1069 SW48 Tot-EGFR HCT116 Tot-EGFR pY774 pY774 Tot-Cbl Tot-Cbl
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Supplemental Fig. 3 - - + + - - + + - - - - + + + + -/Mut WT/- -/Mut
Conditioned media - - + + - - + + EGF - - - - + + + + pEGFR y1125 Hec1A b-actin P-EGFR y1125 DLD1 b-actin
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Supplemental Fig. 4 Hec1A HCT116 ERK1/2 ERK1/2 AKT AKT WT/Mut - /Mut
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Supplemental Fig. 5A DLD1 HCT116 % confluency Hec1A Time (hours)
No siRNA NTC KRas HCT116 % confluency Hec1A Time (hours)
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Supplemental Fig. 5B DLD1 % wound closure Hec1A Time (hours) No siRNA
NTC siKRas No siRNA NTC KRas DLD1 % wound closure Hec1A 0 h Time (hours) 24 h
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Supplemental Fig. 6
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Supplemental Fig. 7 DUSP5 DUSP6 mutKRas WT KRas mutKRas WT KRas ETV1
Relative expression mutKRas WT KRas mutKRas WT KRas ETV1 ETV5 Relative expression ND ND mutKRas WT KRas mutKRas WT KRas
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NT5E IER3 mutKRas WT KRas mutKRas WT KRas UPP1 mutKRas WT KRas
Relative expression mutKRas WT KRas mutKRas WT KRas UPP1 Relative expression mutKRas WT KRas
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Supplemental Fig. 8 A B C KRas NT5E (normalized to NTC) Expression
ETV1 (normalized to NTC) Expression
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Supplemental Fig. 8 1 1 1 D E p=0.0006 p=0.026 p=0.227 p=0.108 H838
1.2 p=0.18 1 1 1.2 H661 H1299 1.0 1.0 RKO HT55 0.8 0.8 H1568 Viability (Target gene:NTC) A427 Viability (Target gene:NTC) 0.6 0.6 A549 HupT3 0.4 0.4 H358 MiaPaca 0.2 0.2 KRas WT mut WT mut WT mut KRas WT mut WT mut WT mut siRNA KRas NT5E ETV1 siRNA KRas NT5E ETV1
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