1. What are the two requirements of chemiosmotic hypothesis?
What molecule is responsible for creating the H+ gradient?

2. What molecule is responsible for creating the H+ gradient?
Ubiquinone
quinone
quinol or
catechol

3. What’s new in this book’s presentation of respiration?
What’s confusing?

4. What’s new in this book’s presentation of respiration?
What’s confusing?
By Mariana Ruiz Villarreal LadyofHats - the diagram i made myself using adobe illustrator. as a source for the information i used the diagrams found here:[1], [2], [3], [4], [5], [6] and [7]., Public Domain,

5. How many proteins are involved?
What are their roles?
Q + 2e- + 2H+  QH2
Q + 2e- + 2H+  QH2
Which metalloproteins have their own names?

6. What is the reaction that provides the energy for ATP formation?
How many and which metals are involved in respiration?
In what types of centers are these metals in?

7. Complex III – also creates H+ gradient while moving along e-
H+‘s are pulled from inside for Q redn
matrix Q
QH2
2 Q
H+ ‘s are released outside from QH2 oxidn
2 x QH2
cytosol c1 c
Cyt c

8. Complex IV – Cytochrome C Oxidase
Fe(II)  Fe(III)
Cu(II)—Cu(I)  Cu(I)—Cu(I)
Cu(II)  Cu(I)

9. Complex IV – Cytochrome C Oxidase
oxy
O-O2-
H+ limiting
Internal
redox
peroxy
reduced
– H2O
ferryl
– H2O
H+ limiting
oxidized
START

11. How do chl molecules behave as attennae?
Photosystem I
SUN CATCHER
Each lobe of clover-leaf-shaped crystal of photosystem I contains 12 different proteins,
shown here in different colors with only the protein backbone of each depicted.
Amid the proteins are 96 chlorophylls (pale yellow) as well as other cofactors.

12. What are the two processes in PS and what are their names?
carboxylation
regeneration
reduction
Outside thylakoid:
4 H+ lost from here
Inside thylakoid:
4 H+ produced here
What is the OEC?

13. H2O  PSII plastoQ  cyt b6f  plastocyanin  PSI  Fe2S2  NADP+
carboxylation
regeneration
reduction
Outside thylakoid:
4 H+ lost from here
Inside thylakoid:
4 H+ accumulates here
Net ET reaction:
H2O  PSII plastoQ  cyt b6f  plastocyanin  PSI  Fe2S2  NADP+

14. The magical partnership of Metal and organic redox
Ubiquinone
Stigmatellin - co-crystallized in Cyt B1C

15. “Z-Scheme”
How is the energy of light converted into chemical energy in the 1st step?
How many and which metals are involved in PS?

16. Eox (V) +1.2 V +0.6 V 0.0 -0.6 V -1.2 V + 4e- PSI parallels
Complex IV= cyt c oxidase in respiration
Plastocyanin in PSI
parallels cyt c in respiration
+ 4e-
Mn4O4 cluster in PSII
parallels Complex I in respiration

19. confusing?

20. What are the similarities and differences between respiration and photosynthesis?

21. Cristae and thylakoid: inner membrane structures
Cyt bc in mitochondria ~ cyt b6f in chloroplasts
Small organic electron/proton carriers
Multi-metal site for multi electron processes
Reactive centers embedded in membranes
Creation of proton gradient
PSII reduces a Q to QH2; PSI is like complex IV
Plastocyanin functions like Cyt c
Use of aa residue as radical (Tyr)

22. 3.5 Å resolution

24. Cytochorme b6f (analog of respiratory cytochrome b1c)

25. PSI Reaction Center
cofactors only

26. PSI Reaction Center E-
acceptor
Chl
pigments