1. B) A) ** ** ** **
Cell count
(h)
Figure S2. Early loss of mitochondrial transmembrane potential and time course of apoptosis induction following ATL treatment in Z-138 cells.(A) Cells were untreated (-) or treated with 10 μM (-) perifosine or (-) edelfosine for 6 h, and loss of mitochondrial transmembrane potential (ΔΨm) was measured as described in Materials and methods using DiOC6(3). (B) Cells were treated with 10 μM perifosine or edelfosine for the indicated times, and then apoptosis was quantitated as the percentage of cells in the sub-G0/G1 region following cell cycle analysis by flow cytometry. Data shown are means ± SD or representative experiments of three independent experiments. Asterisks indicate values that are significantly different from untreated control cells at p < 0.01 (**) level by Student’s t-test.