1. Wastewater analysis: source of information about the relation between frequently abused illicit drugs and music genre preferences
Paula Brandeburová, Marianna Czölderová, Igor Bodík, Roman Grabic, Tomáš Mackuľak

2. The autors declare that they have no conflict of interest

3. Aims of the study
We analyzed 26 illicit drugs and pharmaceuticals in wastewater during seven Slovak and Czech music festivals
The number of monitored participants was over
Monitoring involved 7 specific music genre festivals - metal, rock, pop, country, folk, dance, trance
Comparison of illicit drugs consumption in relation to the specific music genres

4. Monitoring of drugs and psychoactive substances in wastewater
allows one to monitor drug utilization in dependence on time (weeks, months, years)
determine drugs consumption trends, compare quite accurately drugs usage among individual cities, regions, and countries or in relation to the specific music genres

5. Descriptions of the music festivals included in the study
Festival Pohoda (SR - Trenčín) – multicultural festival, cca participants
Grape festival (SR - Piešťany) – dance music, participants
Festival Hudba na Mlynoch (SR - Bratislava) – multicultural festival, participants
Topfest (SR - Piešťany) – rock and metal music, participants
Festival Lodenica (SR - Piešťany) – traditional country festival, about participants
Guláš fest (CZ – Valašské Meziříčí) – folk and country music, more than participants
Vandal fest (CZ - Zubří) – metal festival, about participants

6. Characterization of the WWTPs
Country
WWTP
Number of inhabitants
Number of inhabitants during the festival
Inffluent [m3/d]
Number of samples
Lenght of sewer
[km] SR
Bratislava 7
1 – 30
Trenčín
47 000
63 000
8 500 8
1 – 10
Piešťany
30 000
50 000
16 200 12
3 – 10 ČR
Valašské Meziříčí
23 000
6 400 6
1 – 15
Zubří
25 000
10 100
Overall 39
8 – 86

7. Map of analyzed WWTPs in SR

8. Consumption of legal drugs – caffeine, nicotine (SR)

9. Consumption of illicit drugs in Slovakia and Europe (based on wastewater analysis)

10. EMCDDA (2016)

11. EMCDDA (2016)

12. EMCDDA (2016)

13. Sampling and analysis
Sampling - 24-hour composite samples from three Slovak and two Czech waste water treatment plants (WWTPs) were collected
Analyzed were 26 psychoactive compounds, illicit drugs and their metabolites with LC – MS/MS method

14. Results Results – reported in mg/d per 1000 inhabitants (load)
The most dominant drug was methamphetamine
The most significant relationship between illicit drug use and music genre - stimulants (ecstasy, cocaine) during the dance music festival

22. Conclusions
In the study, we monitored 26 psychoactive compounds in wastewater from 5 cities during the 7 different music genres festivals
Dominant used illicit drug was methamphetamine
Significant relationship between drug use and musical genre was in the case of stimulants (ecstasy, cocaine) use - dance music festival

23. Thank you for your attention!
Paula Brandeburová
Marianna Czölderová
Igor Bodík
Roman Grabic
Tomáš Mackuľak
Thank you for your attention!
This work was supported by the grant scheme for supporting young researchers under the conditions of STU in Bratislava: „Nanomateriály – inovatívny postup odstraňovania mikropolutantov“ and grant scheme for supporting excellent teams of young researchers under the conditions of STU in Bratislava: „Mikropolutanty a rezistentné kmene baktérií ich monitoring a možnosti použitia inovatívnych postupov na ich odstránenie – nanomateriály a železany“.

24. Invitation to our poster