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Published byCameron Richardson Modified over 6 years ago
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Thermophilic spore-forming spoilage organisms
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Organisms and their D-values
Public health concern; Clostridium botulinum and perfringens, Bacillus cereus; D100C ~ 7-30 min Mesophilic aerobes; several Bacillus species including subtilis, licheniformis, megaterium, Alicyclobacillus acidoterrestris; D100C ~ min Thermophilic aerobes; Bacillus coagulans Geobacillus stearothermophilus; D100C ~ min Mesophilic anaerobes; C. tyrobutyricum, butyricum, and sporogenes; D100C ~ very small (C.t and C.b) up to min (C.s) Thermophilic anaerobes; Desulfotomaculum nigrificans and C. thermosaccharolyticum; D100C ~ min
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Endospores Great site to learn more:
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Characteristics of endospores
Business end: Low moisture Proteins protecting DNA DNA repair enzymes Endospore stain
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Alicyclobacillus Spore forming, gram negative, acidophilic
Also grows down to pH 2.5 Heat resistant (D95C ~ 2.6 min) Found throughout juice production environment Produces guaiacol (2-methoxyphenol) Medicine, smoky, hammy, disinfectant-like
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Code of Federal Regulations = CFRs
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