1. Molecular Axon Guidance
PC12 cell….
then NGF added
NGF is neurotropic and neurotrophic

2. Guidance Factors
Growth Factors

4. Netrin..was one of the first neurotropic factors

5. Findings

6. Netrin from FP

7. Netrin from FP,
But perpendicular

8. Netrin in media

10. Cell locomotion like GCs
Cell moves forward by extending filipodia & lamellipodia
Focal adhesions are formed
Cell is pulled forward

14. Diffusible
Contact dependent

17. Extracellular matrix

18. Extracellular matrix
Proteins of ECM - collagen

19. Extracellular matrix
Proteins of ECM - proteoglycans

20. Proteins of ECM - fibronectin
Extracellular matrix
Proteins of ECM - fibronectin

21. Proteins of ECM - laminin
Extracellular matrix
collagen
Proteins of ECM - laminin
laminin

22. Interaction of cells with ECM
INTEGRINS
inactive

23. Interaction of cells with ECM
INTEGRINS
ligands have RGD motif
differentiation
motility
growth
survival
functions of outside-in signals

24. Interaction of cells with ECM
FOCAL ADHESIONS FA

25. Interaction of cells with ECM
FOCAL ADHESIONS

26. CELL ADHESION MOLECULES
Interaction of cells with cells
CELL ADHESION MOLECULES

27. Interaction of cells with ECM and other cells
CELL ADHESION MOLECULES VS
ECM ATTACHMENT MOLECULES

30. Extracellular Ca 2+ Space Wnt E-Cadherin Frz II b-catenin p120
Dsh
Frz II
E-Cadherin
p120 P
b-catenin
actin
filaments
a-catenin
Gsk3b
APC
Axin P
Tcf/Lef

31. Cadherins as Master Regulators of Morphology
Focus is often on adhesive function: Cadherins as molecular glue
Motility
Passive Inhibition
Rho-GTPases
Active Promoter
Adhesion
AJ’s
Type of Cadherin
Stabilize others junctions
Proliferation
CDKI: p27/p21
Wnt
Cadherins
Receptor
Signaling
EGF
FGF
PDGF
Transcriptional/
Nuclear Role
-catenin/Wnt
P120/kaiso
In nucleus
Actin Cytoskeleton
-catenin
Rho-GTPases
Arp2/3