1. Josh Roberts Mia Bawale Lisa Fortin Noah McBride Max Menke Victor Tran
Privacy Committee
Josh Roberts
Mia Bawale
Lisa Fortin
Noah McBride
Max Menke
Victor Tran

2. PCR and Sequencing

3. What is PCR?
Polymerase Chain Reaction allows scientists to reproduce millions of the strands of DNA
It’s done by:
Adding primers, nucleotides, and DNA polymerase to the DNA strand
Thermal Cycles will activated the mass production by denature, anneal, and extension

4. Sequencing
Determining the precise order of the nucleotides of a DNA strand
Next Generation Sequencing
Uses an old technique called Sanger chain-termination
Cluster Generation -Glass slides(Frag), Polymerase, Bridge Amplification
Sequencing - Indexes, Primers, Nucleotides
Data Analysis - Match up with similar reads calls by electrophoresis phohroreises .

5. Case Study - Henrietta Lacks

6. Who was Henrietta Lacks
was born in roanoke virginia 1920
Was poor black and uneducated
Died at 31 due to cervical cancer 1951
had 5 kids before she died

7. HeLa cells Cells could replicate and thrive in a lab
Have been the subject of more than 74,000 studies
Used to create: polio and other vaccines, cancer therapies, in vitro fertilization techniques, and drugs for herpes, leukemia, influenza, hemophilia and Parkinson’s disease

8. Ethical Concerns
The family was not told of the use of Henrietta's cells until 1973
Neither Henrietta nor her family consented to the use of her cells
Her family has received none of the billions of dollars made through the use of her cells
The complete genome of Henrietta was published and made available for download

9. Case Study - DNA Banking

10. What is DNA banking?
DNA Banking is the secure, long term storage of an individual's genetic material.
DNA banks are used to help fight crime
All 50 states have approved DNA banking for use in forensics

11. The benefits of DNA banking
Helps catch criminals
Helps those who have been wrongly accused

12. Disadvantages of DNA banking
Infringes on civil liberties
Could potentially cause discrimination
Revokes criminals right to be rehabilitated
Individual's right to privacy

13. Philosophy of Privacy

14. Pro Aristotelian Justice - Using to the Best Purposes
Henrietta Lacks Case Study:
Using DNA for its purpose: storing human information
Many scientific discoveries were found by using Lack’s cells
Access to more DNA could lead to more discoveries

15. Pro Maximizing Happiness
Producing the most happiness for the majority of society
Case: Wild Goose Chase
Citizens are happier when they feel safe
With more criminals off the street the safer the streets are

16. Con Human Nature “We become individuals through the discovery that we
We all have something to hide, it’s in our human nature
DNA is just genetic makeup does not determine personality traits
We lose sense of our individuality when everyone knows everything
“We become individuals through the discovery that we
could hide something from others”

17. Con J.S. Mill’s Individual Rights
Individual rights and liberty will produce the most happiness in the long run
Case: Wild Goose Chase
Possibility of wrongly prosecutions
With government having access to personal information citizens can feel violated - 4th amendment

18. Website