1. Fig. 1 Expansion of porcine cumulus cells
Fig. 1 Expansion of porcine cumulus cells. A) Compact OCC and B) OOX before culture. FSH stimulated full expansion of OCC (C) and OOX (D) after 24-h culture in medium supplemented with FCS. Sensitivity to Streptomyces hyaluronidase was demonstrated in both cases (OCC, E; OOX, F) as the mucified cumulus was dispersed after treatment with the enzyme. Bar = 50 μm.
Oocytectomy Does Not Influence Synthesis of Hyaluronic Acid by Pig Cumulus Cells: Retention of Hyaluronic Acid After Insulin-Like Growth Factor-I Treatment in Serum-Free Medium1
Biol Reprod. 1999;61(3): doi: /biolreprod
Biol Reprod | © 1999 by the Society for the Study of Reproduction, Inc.

2. Fig. 2 HA synthesis by intact OCC and by OOX during 24 h in the presence of FCS, FSH, and [³H]glucosamine hydrochloride. Controls were complexes cultured in the absence of FSH. Labeled HA retained within the complexes and total HA (complexes plus medium) were measured. Data represent the mean ± SEM from at least five independent experiments, signifying that at least 50 complexes (OCC or OOX) were used per treatment. There were no significant differences between OCC and OOX in all groups (p > 0.05).
Oocytectomy Does Not Influence Synthesis of Hyaluronic Acid by Pig Cumulus Cells: Retention of Hyaluronic Acid After Insulin-Like Growth Factor-I Treatment in Serum-Free Medium1
Biol Reprod. 1999;61(3): doi: /biolreprod
Biol Reprod | © 1999 by the Society for the Study of Reproduction, Inc.

3. Fig. 3 FSH stimulated HA synthesis by intact OCC during 24 h in the presence of either FCS or PVP. HA retained within the complexes or total HA (complexes plus medium) was measured. Data represent the mean ± SEM from at least five independent experiments, signifying that at least 50 OCC were used per treatment. Bars with different superscripts are significantly different (p < 0.01).
Oocytectomy Does Not Influence Synthesis of Hyaluronic Acid by Pig Cumulus Cells: Retention of Hyaluronic Acid After Insulin-Like Growth Factor-I Treatment in Serum-Free Medium1
Biol Reprod. 1999;61(3): doi: /biolreprod
Biol Reprod | © 1999 by the Society for the Study of Reproduction, Inc.

4. Fig. 4 Total and retained HA synthesized by intact OCC and by OOX complexes during 24 h in response to FSH in serum-free, PVP-supplemented medium. Data represent the mean ± SEM from at least five experiments, signifying that at least 50 complexes (OCC or OOX) were used per treatment. There were no significant differences between intact and OOX complexes in their ability to synthesize HA (p > 0.05).
Oocytectomy Does Not Influence Synthesis of Hyaluronic Acid by Pig Cumulus Cells: Retention of Hyaluronic Acid After Insulin-Like Growth Factor-I Treatment in Serum-Free Medium1
Biol Reprod. 1999;61(3): doi: /biolreprod
Biol Reprod | © 1999 by the Society for the Study of Reproduction, Inc.

5. Fig. 5 FSH stimulated HA synthesis by intact OCC cultured for 24 h in medium containing either FCS, or IGF-I+PVP, or PVP alone. Data represent the mean ± SEM from at least five independent experiments, signifying that at least 50 OCC were used per treatment. There were no significant differences between FCS-supplemented and IGF-I-supplemented medium in synthesis or retention of HA by OCC (p > 0.05). Bars with different superscripts are significantly different.
Oocytectomy Does Not Influence Synthesis of Hyaluronic Acid by Pig Cumulus Cells: Retention of Hyaluronic Acid After Insulin-Like Growth Factor-I Treatment in Serum-Free Medium1
Biol Reprod. 1999;61(3): doi: /biolreprod
Biol Reprod | © 1999 by the Society for the Study of Reproduction, Inc.