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cDNA Synthesis Service

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Presentation on theme: "cDNA Synthesis Service"— Presentation transcript:

1 cDNA Synthesis Service
Date: cDNA Synthesis Service Source of RNA 1-1. Sample Information: M1, 갯지렁이 Total RNA 1-2. Total RNA Concentration: NanoDrop® ND-1000 UV-Vis Spectrophotometer Tube # Name Conc. (ng/ul) A260/A230 A260/A280 Total vol. (ul) Total quantity 1 M1 Total RNA -1 639.05 0.96 1.85 1200 ~ 0.766mg 2 M1 Total RNA-2 690.62 0.85 1.83 ~ 0.828mg 3 갯지렁이 Total RNA-1 1.44 2.01 1000 ~ 2.244mg 4 갯지렁이 Total RNA-2 1.42 2.00 ~ 2.425mg Electrophoresis of RNA Lane Sample name Quantity 1 Positive Control 1.0 g 2 M1 Total RNA -1 3 M1 Total RNA-2 4 갯지렁이 Total RNA-1 5 갯지렁이 Total RNA-2 28S RNA (4.8 kb) 28S RNA (4.8 kb) 18S rRNA (1.8 kb) 18S rRNA (1.8 kb) 3. mRNA Isolation Tube # Name Conc. (ng/ul) A260/A230 A260/A280 Total vol. (ul) Total quantity 1 M1 Total RNA 53.02 1.69 1.85 30 ~1.590 ug 2 갯지렁이 Total RNA 948.47 2.55 2.06 ~ ug - 1 / 2 -

2 cDNA Synthesis Service
Date: cDNA Synthesis Service 4. Synthesis of ds cDNA Tube # Name Conc. (ng/ul) A260/A230 A260/A280 Total vol. (ul) Total quantity 1 M1 Total RNA 70.30 2.37 1.70 50 ~ ug 2 갯지렁이 Total RNA 402.51 2.56 1.88 ~ ug Electrophoresis of ds cDNA 1kb Ladder Lane Sample name Quantity 1 Positive Control 50ng 2 1kb Ladder 3 M1 Total RNA 100ng 4 갯지렁이 Total RNA 200ng - 2 / 2 -


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