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Published byRoland Rudolph Manning Modified over 6 years ago
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Endometrial bleeding in hormone replacement therapy users: preliminary findings regarding the role of matrix metalloproteinase 9 (MMP-9) and tissue inhibitors of MMPs Martha Hickey, M.D., Jenny Higham, M.D., Mark Sullivan, Ph.D., Lyn Miles, Ian S Fraser Fertility and Sterility Volume 75, Issue 2, Pages (February 2001) DOI: /S (00)
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Figure 1 Diagram of subcloning required to produce the probes for this investigation, showing the different procedures for TIMP-1 (A) and MMP-9 (B). Hickey. Endometrial bleeding in HRT recipients. Fertil Steril 2001. Fertility and Sterility , DOI: ( /S (00) )
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Figure 2 RNA sequences used for the in situ hybridization procedures. They are complementary to the mRNA sequences and are printed in the 5′-3′ orientation. Hickey. Endometrial bleeding in HRT recipients. Fertil Steril 2001. Fertility and Sterility , DOI: ( /S (00) )
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Figure 3 In situ hybridization for MMP-9 (A) and (B) TIMP-1 in control tissues. Positive cells are indicated by arrows, demonstrating discrete staining. This would be classified as 2 in both cases (weak, localized immunostaining). Original magnification: A, ×100; B, ×200. Hickey. Endometrial bleeding in HRT recipients. Fertil Steril 2001. Fertility and Sterility , DOI: ( /S (00) )
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