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Volume 56, Issue 4, Pages (October 1999)

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Presentation on theme: "Volume 56, Issue 4, Pages (October 1999)"— Presentation transcript:

1 Volume 56, Issue 4, Pages 1282-1285 (October 1999)
Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts  Farhad R. Danesh, Minghao Ye, Sasan Salmi, Michael LaPointe, Daniel Batlle  Kidney International  Volume 56, Issue 4, Pages (October 1999) DOI: /j x Copyright © 1999 International Society of Nephrology Terms and Conditions

2 Figure 1 [3H]-thymidine incorporation per well (A), and [14 C] leucine incorporation per well (B), in fibroblasts from controls (○), IDDM patients without nephropathy (•), and IDDM patients with nephropathy (♦) after 6, 12, and 24 hours of serum stimulation with 10% serum. [3H]-thymidine incorporation and [14C] leucine incorporation increased in the three groups over time as determined by a two-way analysis of variance: [3H]-thymidine incorporation P < 0.001, and [14C] leucine incorporation P < From Lurbe et al[1]; used with permission. Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions

3 Figure 2 Percentage increase in number of the cells in the S phase after serum stimulation based on FACS analysis in five control subjects. Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions

4 Figure 3 Temporal profile of serum-induced cyclin D1 protein expression in cultured skin fibroblasts from a control subject. Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions

5 Figure 4 Temporal profile of serum-induced pRB phosphorylation.
Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions

6 Figure 5 A representative experiment of the temporal profile of serum-induced cyclin D1/CDK4(6) kinase activity. Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions

7 Figure 6 Temporal profile of serum-induced cyclin D1 and pRB phosphorylation in skin fibroblasts from five control subjects. Asterisks denote a statistically significant change (P < 0.05). Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions


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