1. بسم الله الرحمن الرحیم

2. Plant tissue culture

3. Definition of Plant tissue culture : Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition.

4. Brief History of plant tissue culture  Schelden and schwann make toti potency theory in 1838.  Successful Leaf culture by Haberlandt in 1902.  White first time established successful root culture of tomato in 1937.  Skoog and Miller demonstrated the rule of axin and cytokinin on root and shoot formation in tobacco – tissue in 1957.  Edward cocking enzymatic isolation and culture of protoplast 1960.  Murashige and Skoog introduced the medium for tobacco culture in 1962. Gottlieb Haberlandt Born28 November 1854 Mosonmagyaróvár Died30 January 1945 (aged 90) Berlin NationalityAustria FieldsBotany

5. Tissue culture’s purposes Production of Disease free plants True to type In short time In Small place With Low staff Anytime production

6. Type of cultures in Plant tissue culture Plant tissue culture Stem culture Seed cultureMeristem culture organ culture leaf culture Callus culture Bud culture Embryo culture

7. Stem culture Explants collection Washing Sterilizing Culturing

8. Bud culture Explants collection Washing Sterilizing Extraction Culturing

9. Embryo culture Explants collection Sterilizing Extraction Culturing

10. Leaf culture Explants collection washing Sterilizing Culturing

11. Meristem culture(Micrografiting) Explants collection washing Sterilizing Extraction

12. Contin.. Meristem Isolation

13. Continue Micro grafting

14. Seed culture washing Sterilizing Culturing

15. Gisela 5 (rootstock for sweet cheery) GF677 ( rootstock for almond) Pyro dwarf ( rootstock for pear) Rootstock production in plant tissue culture section of PBTL

16. Gisela 5 Its Shoot proliferation have good result in DKW media with 0.5 mg/L BAP and 0.1 mg/L IBA for the root production MS half concentration Macro elements and half concentration sucrose with 0.6 mg/L IBA is perfect.

17. DKW media(Dampf-Kraft-Wagen )

18. GF677 Its Shoot proliferation have good result in GNH media with 0.1 mg/L BAP for the root production MS half concentration Macro elements and half concentration sucrose with 0.6 mg/L IBA and 4 mg/L thiamin HCl is perfect.

19. GNH(Garoosi, Nezami and Haddad)

20. Pyro dwarf Its Shoot proliferation have good result in QL media with 0.8 mg/ BAP/L and 0.08 IBA/L for the root production MS half concentration Macro elements and half concentration sucrose without hormone

21. QL(Quoirin and Lepoivre)

22. Thanks from your attention Question?