1. Useful Materials/Procedures to pack proteoliposome samples in 3
Useful Materials/Procedures to pack proteoliposome samples in 3.2 and 4 mm MAS rotors (Anna De Angelis, 2016)
Precision balance- use it to measure the amount of sample that goes in the rotor to make sure it is not overfilled.
Use positive displacement pipettes (any brand), they use a plunger tip to push viscous or sticky samples.
Sharpie- use to mark the position of the bottom of the cap on the outside of the rotor, to make sure it is not overfilled.
For mm rotors, add 5-10 uL of sample at a time, and pack after each addition by spinning down the rotor in a bench-top centrifuge. Before capping the sample, it should be spun down in a swinging bucket centrifuge to ensure a flat meniscus.
Use the instructions on the next page if your swinging bucket centrifuge does not have a small-vial insert.
Clean edge of rotor before inserting caps, to ensure it is not slippery from the lipids.

2. Spinning down a sample in a 3
Spinning down a sample in a 3.2 mm rotor with a 15-ml swinging bucket centrifuge (aka, a home-built adaptor)
1. Fill bottom of eppy with kimwipe
2. Roll kimwipe around rotor to keep it centered upright
view from the side
view from the top
3. Cut away eppy cap
4. Use kimwipe to hold eppy centered upright in a 15 mL conical tube
Note: Have tweezers handy to remove eppy, paper, or rotor
5. Spin down at 5,000 rpm, 20 Celsius