1. WP6 Patho-Stick and Patho-doc development and prototyping
General Assembly Meeting
WP6 Patho-Stick and Patho-doc development and prototyping

2. Objectives Design, blueprint and prototyping : Validating functions
Patho-stick (UCBL)
Patho-doc (Bee)
Validating functions
Preparation of the pre-industrial specifications

3. Period achievements Two technical meetings
Patho-stick: phone conference BEE, UCBL
 Technical_Meeting_Stick_phone_
Extraction: in Molzyme premises (Bremen) MOL, UCBL
 Technical_Meeting_Extraction_Bremen_21_
List of requirements for stick and doc
Blueprint of patho-doc
Prototyping patho-stick

4. List of requierements (D6.1)
Parameter
Options
Impact
Status
DNA extraction systems
Blood volume used
1mL
Size of patho-doc, volume of reagents, sensitivity
1 mL
Plastic for consumable
Printed or molded
Price/material/number of vials
Printed but transferable to molding.
Polyacrylate for printing.
Use of the patho-stick for which sample
Blood, urine, Broncho washing, feaces
From Swap.
Sampling, Extraction, Reagent
Blood for development steps, urine in a second time
NO SWAP
Assay design
Quantitative approach
YES/NO
All WP
YES
Detection principle
Steps and detection tools
Particles, colorimetry, fluorescent
Colorimetry (enzyme or particles)
Amplification
Classical PCR or isothermal
Patho-doc functions
Isothermal
To be confirm by AIT
Surface chemistry
Glass, polystyrene
Material availability, probe modification
Polystyrene

5. List of requierements (D6.1)
Patho-stick
Compatibility between stick configuration for extraction and assay
YE/NO
Complexity of production
YES, the same stick configuration shall be used for extraction and assay. Only changing volumes and reagents
Size
10-100mm
Printing technology
15cm*5cm*5cm (500mL bottle size)
3D organization
Flat
Layered
Assembly
Either
Stick pre-filled
Liquid reagent
Solid reagents
Valves and protocol
Yes, as possible
Both maybe
Array measurement
In the stick
Outside the stick
Transparency of at least one material
Integrated measurement
Detector in the stick or in the doc
Technological complexity of the stick
Optical detector on the patho-doc
Size of the array
Permit naked eye reading
200µm with 600µm pitch at least
Price of the patho-stick
Material cost around 2€-20€.

6. List of requierements (D6.1)
Patho-doc
Footprint
Patho-doc footprint is likely to be approximately 300x350mm
Pipetting
Which volume
Type of liquid handling system involved
assay and extraction shall work using 0.5-1mL range volume
Function to be implemented
Pipetting, heating, cooling, lightning, filtration, imaging…
Complexity of the machine
Heating 18°C-90°C (hopefully isothermal)
Imaging
Moving parts (rotating device is foreseen)
Filtrate under vacuum (600 mBar)
Sterilization
Possibility of machine sterilization
Materials used
YES, UV is foreseen
Waste container
In the stick or bellow
Size of the stick
Bellow (12mL) To be confirmed.

7. List of requierements (D6.1)
Prototyping duty
Patho-stick for extraction and assay production and prototyping duty
UCBL and MOL for determination of extraction protocol
UCBL and Bee for prototype models
UCBL for prototyping

8. Blueprint patho-doc Patho-doc & Patho-Stick Conceptual designs
Compact instrument
Suitable for running both Extraction and Micro-Array protocols
Simple interface
Connection to external PC for interpretation of results

9. Blueprint patho-sticks
Patho-doc & Patho-Stick Conceptual designs
Patho-Stick (Extraction)
Moulded carousel component for 1ml extraction protocol.
Positions for reagents and buffers to process single sample.
Heated position for mixing (W0).
Vacuum cartridge is separate to patho-stick.
Patho-Stick (Micro-Array Assay)
Separate moulded carousel component
Heated reaction plate position.
Reaction plate to be a separate transparent moulded part, suitable for imaging.

10. Blueprint patho-doc Patho-doc & Patho-Stick Conceptual designs
5 Axis instrument
Proven linear actuators and drives
Stepper motor control

11. Blueprint patho-doc Patho-doc & Patho-Stick Conceptual designs
Conceptual design work is ongoing.

12. Patho-stick prototyping
One stick for assay
One stick for extraction
One through for Patho-Robot