1. From: Zeb1 Represses Mitf and Regulates Pigment Synthesis, Cell Proliferation, and Epithelial Morphology
Invest. Ophthalmol. Vis. Sci ;50(11): doi: /iovs
Figure Legend:
Sphere formation reprograms dedifferentiated RPE cells back to a differentiated phenotype. (A, left) A cluster of wild-type RPE cells at day 1 in culture. Middle: wild-type RPE cells after 1 week in culture. Note the appearance of nonpigmented cells with fibroblastic morphology at the edge of the pigmented cells. Right: higher power view of the edge of the pigmented cells after 1 week in culture. (B, left) Wild-type RPE cells at passage 8. Middle: passage 8 RPE cells at day 1 as spheres in suspension culture. Right: feeder layer cells. (C, left) RPE spheres bound to the feeder layer. Middle: RPE cells from spheres are shown migrating onto the feeder layer (day 3). Right: higher power view of the boxed region from the middle panel. (D, left) RPE cells from spheres after migration onto the feeder layer (day 7). Middle: RPE cells from spheres that migrated from the feeder layer onto the surrounding culture dish (day 7). Right: RPE cells from spheres bound to feeder layer cells on Petri dishes after 1 month in culture.
Date of download: 3/9/2018
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