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Published byMaria Ellingsen Modified over 6 years ago
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1-4 Tools and Procedures Key Concepts: Vocabulary:
What measurement system do most scientists use? How are light microscopes and electron microscopes similar? How are they different? Vocabulary: Metric system Microscope Compound light microscope Electron microscope Cell culture
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Microscopes Two main types: Light Microscopes Electron Microscopes
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EYEPIECE COARSE ADJUSTMENT FINE ADJUSTMENT ARM LOW-POWER OBJECTIVE ARM HIGH-POWER OBJECTIVE STAGE STAGECLIPS FINE ADJUSTMENT STAGECLIPS BASE IRIS MIRROR COARSE ADJUSTMENT LAMP DIAPHRAGM
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MICROSCOPE NOTES COMPOUND LIGHT MICROSCOPE: most common scope
Advantages: get higher magnification than with a dissecting scope. Drawbacks: the light comes from below so sample must be very thin so you can see Usually view dead things with this microscope. Limit of resolution: if you magnify beyond this point, the object will be blurry.
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DISECTING MICROSCOPE: used to view dissection or small live (sleeping) animals such as fruit flies.
Advantages: can view samples that are alive; large field of view Drawbacks: magnification is not as high as other microscopes.
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SCANNING ELECTRON MICROSCOPE (SEM): used to see very small “stuff”
Advantages: can study very small objects such as viruses and individual molecules; can magnify things 1000x that of a light microscope. Drawbacks: object must be dead; also very expensive!
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Face of an ant! IMAGES PINHEAD
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