1. High Sensitivity Luminescence Detection
LuminMax-C
for
High Sensitivity Luminescence Detection
Look for potential opportunity for strategic alliance, VC, or initial funding
Maxwell Sensors Inc.
9/16/2018
Maxwell Sensors Inc.

2. Who will use ? Biotechnology research laboratories
University biological and biochemical laboratories
Hospital research laboratories
Government biological and biomedical laboratories
Food industry
Environmental testing
Forensic testing
9/16/2018
Maxwell Sensors Inc.

3. What kind of tests ? Immunoassay and proteomics
Nucleic acids, DNA, RNA, and genomics
Cell viability tests, luciferase, ATP assay
Clinical diagnostics and tests
E-coli bacterial food tests
Toxin environmental tests
Restaurant sanitary tests
9/16/2018
Maxwell Sensors Inc.

4. What is Luminescence ?
- It generates light by chemical reactions, the light signal can be used
for analyte quantification
- It does not require external light source to excite the analyte, such as
fluorescence or absorbance measurement
- Due to photon counting detector can detect a few photons,
the detection can be extremely sensitive
- Common luminescence are chemiluminescence & bioluminescence
9/16/2018
Maxwell Sensors Inc.

5. How the light is generated?
- There are more than 50 chemical reactions have been used to
generate light
- The most common reactions use:
Substrate + Enzyme  excited state molecule*  Light or
Substrate + Enzyme + ATP  excited state molecule*  Light
*excited state molecule: e.g. dioxetane
When the 6M patients show up in the E-room, one of---, to quickly decide should let the patient go home, or admit for surgery or other checkup
9/16/2018
Maxwell Sensors Inc.

6. What are the substrates?
There are many different substrates & their derivatives, such as
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
9/16/2018
Maxwell Sensors Inc.

7. What are the substrates?
There are many different substrates & their derivatives, such as
Luciferin
Acridinium ester
Luminol
Isoluminol
Lumi-PPD,Lumigen-PS-atto,
etc
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
9/16/2018
Maxwell Sensors Inc.

8. What are the Enzymes? Horseradish peroxidase (HRP) Luciferase
Alkaline phosphatase (AP)
Peroxidase
Dehydrogenase
Galactosidase, etc.
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
9/16/2018
Maxwell Sensors Inc.

9. Example 1 Enzyme can be used for quantification
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
Enzyme can be used for quantification
9/16/2018
Maxwell Sensors Inc.

10. Example 2 Enzyme, ATP, or Mg++ (Ca++) can be used for quantification
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
9/16/2018
Maxwell Sensors Inc.

11. How to use for quantification?
Enzyme: can be used for indirect labeling proteins or nucleic acids, so it can be used to quantify proteins or nucleic acids
Luciferase, ATP, or Ca++ can be used for direct quantification of biological cells
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
9/16/2018
Maxwell Sensors Inc.

12. How to label proteins? *: enzyme Light
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
Light
9/16/2018
Maxwell Sensors Inc.

13. Enzyme linked immunosorbent assay
(ELISA)
9/16/2018
Maxwell Sensors Inc.

14. Sandwich & Competitive assay
9/16/2018
Maxwell Sensors Inc.

15. How to label nucleic acids?
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
9/16/2018
Maxwell Sensors Inc.

16. How to label nucleic acids?
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
9/16/2018
Maxwell Sensors Inc.

17. Glow Luminescence
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
9/16/2018
Maxwell Sensors Inc.

18. Flash Luminescence Stable Flash (Promega, Lumigen, etc.)
Troponin is an established and standard markers, but it require sensitivity –
No system meet these requirements
Conventional flash
9/16/2018
Maxwell Sensors Inc.

19. Luminescence Response Time
Lumigen-PS-atto (rapid response)
9/16/2018
Maxwell Sensors Inc.

20. Sensitivity for Cell Analysis
Promega kit
9/16/2018
Maxwell Sensors Inc.

21. LuminMax-C vs. Beckman-LD400
9/16/2018
Maxwell Sensors Inc.

22. LuminMax-C System
9/16/2018
Maxwell Sensors Inc.

23. Luminescent Kits Resources
Promega- reagent kits, cell kits
Stratagene- reagent kits
Bioassay System- luciferase gene assay kits
Pierce- enzymes, substrate
KPL- enzymes, substrates
Lumigen- substrates
etc.
9/16/2018
Maxwell Sensors Inc.

24. Microplate Resources Nunc Costar, Corning, Perkin Elmer,
BD Biosciences,
Greeiner bio-one
Evergreen Sciences
Maxwell Sensors Inc. (low cost strip & reusable tray)
9/16/2018
Maxwell Sensors Inc.

25. Microplate Varieties Types of microplates
Bottom clear: read from bottom (for MSI-LuminMax-C)
Total opaque: need to read from top,
signal depends on solution volume
Types of surface treatment:
Untreated, Sterile
Treated, Sterile – increase binding
Coating for specific proteins or Nucleic acids
9/16/2018
Maxwell Sensors Inc.

26. LuminMax-C Advantages
User Friendly:
Very easy to use, minutes learning will be able to start to
operate.
Cost Effective:
The most cost-effective and high performance system on the world market.
Offers low cost consumables
High Sensitivity:
One of the most sensitive photoncounting luminometer on the market, the system has the ability to detect a few photons or attomoles molecules.
9/16/2018
Maxwell Sensors Inc.

27. LuminMax-C Advantages (conti.)
Extremely Compact: It is suitable for laboratory uses.
Consistent Testing Results:
Signal is sample volume independent.
Minimum Cross-talk:
Unique adapter design offers minimal cross-talk and excellent optical efficiency.
Solid Packaged:
The system is packaged in metal case; it prevents components damage or mis-alignment during shipping.
Excellent service:
Rapid responses (e.g. 24 hours) and immediately actions
9/16/2018
Maxwell Sensors Inc.