1. PML/TRIM19-dependent inhibition of HIV-1 reverse-transcription by Daxx
Jacques DUTRIEUX PhD.
INSERM U941 – Saint Louis Hospital
Paris, FRANCE Contact:
PML/TRIM19-dependent inhibition
of HIV-1 reverse-transcription by Daxx
DUTRIEUX J. et al., PLOS Pathogens 2015
Good afternoon,
I will present you the work I’ve done during my PhD in Rémi Cheynier’s lab at the Institut Cochin in Paris where I’ve studied the impact of acute SIV infection on thymopoiesis in rhesus macaques.
INSERM UMR-S 1124
Team « Mechanism of interferon action and biotherapeutic pathways » Sébastien NISOLE & Mounira K. CHELBI ALIX
Paris, FRANCE

2. Promyelocytic Leukemia protein inhibits HIV-1 infection
Day post-infection
p24 (ng/ml)
shRNA CTR
shRNA PML
Mock
PBMC
MEF
MOI wt
PML KO
PML KO + As2O3
wt + As2O3
GFP-positive cells (%)
HIV-1 infection is enhanced in absence of PML
Uninfected
HIV-1
10 µm
PML
p24
Retroviral infections triggers a transitory formation of PML cytoplasmic bodies

3. Daxx is the protein responsible for HIV-1 restriction
GFP-positive cells (%)
MOI wt
Daxx KO
MEF
Day post-infection
p24 (ng/ml) EV
Daxx
Mock
PBMC
HIV-1 infection is enhanced in absence of Daxx and reduced when Daxx is over-expressed
Mock HIV-1
10 µm
Daxx co-localizes with the incoming HIV-1 capsids
Daxx
Actin
wt PML KO
Sp100
MEF 1
0.5
1.5
Daxx / RPL13A
shRNA CTR PML
shRNA PML
MG132
HeLa
PML is necessary for Daxx expression and stabilization

4. In presence of PML No PML What triggers Daxx degradation ?
What triggers PML CBs formation?
How Daxx inhibit Reverse Transcription ?
What are the constituents of PML CBs ?