1. AML1/Runx1 and oncogenic transformation
RUNT domain (DNA BINDING)
Chr 21
Chr 8
4 Nervy repressor domains
In human myeloid leukemia =>
Runx1/AML1 is a Runt family transcription factor critical for normal hematopoiesis: Runx1 has been identified in leukemia-associated chromosomal translocations, including t(8;21)
Translocation (8;21)
RUNT domain
4 Nervy repressor domains

2. Mcoffee: multiple sequence alignment and phylogenetic tree
RUNX1 alignment
0.1
Lozenge Drosophila
Runt Drosophila
Runx D.rerio
Runx1 G.gallus
RUNX1v2 H.sapiens
Runx1 R.norvegicus
Runx1 M.musculus

3. (Drosophila)
NO alignment between species for the t(8;21)

4. Is there a difference between the proteinic folding
of AML1
and AML1-ETO??

5. Predicted structures of AML1-ETO domains
NCBI- BLAST
RUNT Zf
NRH2 domain
PDB- models database

6. AML1-ETO: hydrophobic cluster analysis with drawhca
Runt
TAFH(NHR1)
NHR2
NHR3
zf-NHR4
Phosphorylation site?

7. Scanning for Phosphorylation-sites of AML1: Scansite
ERK-1

8. Conclusions: Biocomputing study of AML-1 ETO
Sequences comparison vs AML-1
3D prediction
Hydrophobic domains prediction
Potential phosphorylation sites of AML1
Comparison with AML1-ETO

9. .....and now you have to return to the lab and make experiments...........