1. Validating Cdk‐dependent chromatin association changes of selected outliers A–EValidation of changes in chromatin affinity following Cdk inhibition of indicated candidates (2 h, 50 μM roscovitine) using Triton extraction and immunofluorescence (IF) in HeLa cells.
Validating Cdk‐dependent chromatin association changes of selected outliers A–EValidation of changes in chromatin affinity following Cdk inhibition of indicated candidates (2 h, 50 μM roscovitine) using Triton extraction and immunofluorescence (IF) in HeLa cells. Quantification of IF data was performed by measuring changes in signal intensities in Image J (A–D) and qualitative analysis of nucleolar staining (E). In each experiment, n > 50 nuclei were analyzed for each condition in three independent experiments (P indicates P value from Mann–Whitney test).
Georg Kustatscher et al. EMBO J. 2014;33:
© as stated in the article, figure or figure legend