1. Odile Huchette, research engineer
G&H October 2002, Montpellier WP3 / cultivation, P4 P4 : persons involved
Odile Huchette, research engineer
Christèle Bellamy, research technician
Didier Perrin, research assistant
Rémi Kahane, "co-ordinator"
COOPD'OR R&D-CIRAD FLHOR

2. G&H October 2002, Montpellier WP3 / cultivation, P4 Workplan 2002
Milestones 3rd year :
Differential display of clones exposed in vitro to various mineral nutrition treatments
Greenhouse experiment in various S regimes
Sampling of fresh material during the cultivation treatment for CSO analysis (P4 & P5)
Jan Feb. March April May June July Aug. Sept Oct. Nov Dec.
VITRO
Micropropagation nd Micropropagation Experiment with various S
of 4 genotypes nutrition
Experiment with V. Chovelon / L. Fereol : Effect of different factors (light source, photoperiod and medium) on in vitro bulblet production (3 varieties)
Green-house
Greenhouse experiment
COOPD'OR R&D

3. Printanor (PRI), Morasol (MOR), Messidrôme (MES)
G&H October 2002, Montpellier WP3 / cultivation, P Greenhouse experiment 2002
 3 varieties :
Printanor (PRI), Morasol (MOR), Messidrôme (MES)
 3 Sulphur nutrition treatments:
0 (in the macro-elements) = Treatment S-
2 meq (normal) = Treatment TS
8 meq = Treament S++
COOPD'OR R&D

4. 01/03 : Plantation of the cloves
G&H October 2002, Montpellier WP3 / cultivation, P Greenhouse experiment 2002
01/03 : Plantation of the cloves
26/03 : first mineral nutrition (4-5 leaves)
17/06 : Harvest of Messidrôme
10-11/07 : Harvest of Morasol
16-17/07 : Harvest of Printanor
Plantation in boxes with washed river sand
Mineral nutrition with drop irrigation
COOPD'OR R&D

5.  Weekly observations : Growing of the leaves and bulbs
G&H October 2002, Montpellier WP3 / cultivation, P Greenhouse experiment 2002
 Weekly observations :
Growing of the leaves and bulbs
 Measurements at harvest :
- weight of the bulbs
- alliin content (P5)
- total sulfur (P2) in progress
COOPD'OR R&D

6. Bulb growing = diameter of the bulb / diameter of the neck
G&H October 2002, Montpellier WP3 / cultivation, P Growing of the 3 varieties
Bulb growing = diameter of the bulb / diameter of the neck
COOPD'OR R&D

7. G&H October 2002, Montpellier WP3 / cultivation, P4
G&H October 2002, Montpellier WP3 / cultivation, P Weight of the bulbs
COOPD'OR R&D

8. G&H October 2002, Montpellier WP3 / cultivation, P4 Alliin content of the bulbs (P5)
COOPD'OR R&D

9. (nmol / mg fresh matter)
G&H October 2002, Montpellier WP3 / cultivation, P4 Statistical analysis  influence of the variety
S++
MES
MOR
PRI
VARIETY 51 70 89
108
127
(nmol / mg fresh matter)
ALLIIN S- TS
Analysis of Variance
COOPD'OR R&D

10. (nmol / mg fresh matter)
G&H October 2002, Montpellier WP3 / cultivation, P4 Statistical analysis  influence of the S treatment
MES
S++ S- TS
TREATMENT 51 70 89
108
127
(nmol / mg fresh matter)
ALLIIN
MOR
PRI
Analysis of Variance
COOPD'OR R&D

11. G&H October 2002, Montpellier WP3 / cultivation, P4 Milestones Year 3
Differential display of clones exposed in vitro to various mineral nutritions in progress
Greenhouse experiment in various S regimes 
Sampling of fresh material during the cultivation treatment for CSO analysis (P4 & P5) 
COOPD'OR R&D

12. G&H October 2002, Montpellier WP3 / cultivation, P4 Future experiments
In Vitro : Differential display of clones exposed in vitro to various mineral nutritions
Effect of an absence of S in the macro-elements : role of S atmospheric ?
Effect of high values of S in the medium : phytotoxicity?
Interaction between S and light (quality + quantity)
Interaction between S and N, or S and Se ?
COOPD'OR R&D

13. In Greenhouse (2003) : COOPD'OR R&D
G&H October 2002, Montpellier WP3 / cultivation, P4 Future experiments and Milestones Year 4
In Greenhouse (2003) :
Greenhouse cultivation of garlic bulbs enriched in CSO ?
Comparison between extreme genotypes low and high in S content and comparison virus-free and virus-contaminated garlic for sulphur composition CSO content ?
 Greenhouse cultivation of garlic bulbs providing from the experiment in greenhouse 2002
Chemical analysis (P5) before and after processing into powder. Transfer to the health partners ?
At high level of S, effect of the N level (3 mineral nutrition treatments) ?
COOPD'OR R&D