1. Supplemental Data Figure 3: Reverse phase-HPLC of peripheral venous plasma extract from subject 3. Each fraction was assayed with two separate immunoassays: N-terminal assay – solid dark grey line and triangles, and the commercial assay – heavy black line and closed circles. There was insufficient plasma to measure these fractions for C-terminal immunoreactivity. The elution positions of BNP(1-32) and BNP(3-32) standards are shown by the large and small arrows respectively, and that of proBNP by the square bracket. Major peaks have been numbered to correspond with those in the text.