2. OVERVIEW OF HYDROGEN PRODUCTION
Steam reforming - Natural gas /Coal
Thermo chemical metods-Using thermocyclers
France , Germany ,Japan ,USA- countries produces hydrogen
3. Biohydrogen

3. LIMITATION Co production of oxygen
Presence of nitrogenase ( oxygen sensitive enzymes)
Continuous production of hydrogen
Unavailability of commercial production methods.

5. Photosynthetic bacterial hydrogen production
Cyanobacterial hydrogen production
- Atmospheric condition
Photosynthetic bacterial hydrogen production
-media composition , identification
- light ,substrate, atmospheric condition.
Nitrogen
Argon
Methane
CO2

6. Co- cultivation of Cyanobacteria and photosynthetic bacteria
Designing photo-bioreactor for large scale continuous production

7. Light duration - 24 hours [ 18/6] total volume - approx. 13 ml vials
Conditions :
Temperature - 30°C
Light Intensity lux
Light duration - 24 hours [ 18/6]
total volume - approx. 13 ml vials
culture volume - 5 ml

8. NITROGEN ATMOSPHERE

9. ARGON ATMOSPHERE

10. METHANE ATMOSPHERE

11. Pink color
Yellow color
Dark brown color
Green color
Identification for genus and species.

12. Solution I Solution II SolutionIII
g/L
Trace metal mix
mg/L
Potassium di-hydrogen phosphate
0.33
mg/L
Zinc sulfate 10
Ferrous sulfate
200
Ammonium chloride
0.5
Manganese chloride 3
Magnesium chloride 3
NOTE
1ml of Solution II & 0.5ml of Solution III were added to the media after autoclaved.
Boric acid 30
Sodium chloride 25
Cobaltous chloride 20
Calcium chloride
0.05
Cupric chloride 1
Sodium succinate
1.0
Nickel chloride 2
Yeast extract
0.02
Sodium molybdate 3
Agar 25 pH
3-4 pH 7

13. 1.Purple non sulfur bacteria
2. Green sulfur bacteria