1. An analysis of Plk4 centriolar dynamics.
An analysis of Plk4 centriolar dynamics. (A) Micrographs show spinning-disk confocal fluorescence images taken from a time-lapse movie of an early embryo expressing Jupiter-mCherry (as a microtubule marker) and Plk4-GFP; time in minutes:seconds is indicated. At time t = 0:00, the embryo is in early S-phase, and the centrosomes have recently separated. Inset shows a single centriole pair (bar, 1 µm), which is then shown at 30-s or 1-min intervals (bar, 0.5 µm). The cell-cycle stage is indicated above each time interval. A series of lower-magnification views of the embryos are shown below the images of the individual centrioles to illustrate how MTs were used to determine the cell-cycle stage at each time point. Bar, 10 µm. (B) Graph shows the mean Plk4-GFP (brown) incorporation profile during S-phase of nuclear cycle 12 in WT embryos (n = 6 embryos). The mean incorporation profile of Sas-6-GFP is shown overlaid (dotted orange, same data shown in Fig. 3 A, but normalized for the length of S-phase). (C and D) Charts quantify parameters of Plk4-GFP behavior derived from mean Plk4-GFP profiles from embryos at nuclear cycles 11, 12, and 13 (n ≥ 5 embryos for each cycle; n = 34, 31, and 33 centriole pairs [mean] per embryo, respectively). A.U., arbitrary units. Data are represented as mean ± SD. Statistical significance was assessed using either an ordinary one-way ANOVA test (for Gaussian-distributed data) or a Kruskal–Wallis test, and is shown above each chart.
Mustafa G. Aydogan et al. J Cell Biol 2018;217:
© 2018 Aydogan et al.