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Supplementary Figures (Molecular Breeding)
Epigenetic modification in rice controls meiotic recombination and segregation distortion Yoshiki Habu, Tsuyu Ando, Sachie Ito, Kiyotaka Nagaki, Naoki Kishimoto, Fumio Taguchi-Shiobara, Hisataka Numa, Katsushi Yamaguchi, Shuji Shigenobu, Minoru Murata, Tetsuo Meshi, Masahiro Yano Corresponding author: Yoshiki Habu, Agrogenomics Research Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba , Japan. Phone: , Fax: ,
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Supplementary Fig. S1 + - (a) ACTIN RCS2 RT forward reverse RT primer
wt asDDM1 RT primer (b) (c) F1(NB x KS) F1(NB x KS) F1(asDDM1 x KS) TSA 1 2 3 4 5 - 1 2 3 4 5 Supplementary Fig. S1
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Supplementary Fig. S2 chromosome 3 chromosome 11 (a) (b) 20 15
recombination repressed region in the wild type CENH3 region 15 average fold 10 gap in the centromeric region 5 Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g Os03g asDDM1 - specific recombination region (b) chromosome 11 20 recombination repressed region in the wild type 15 average fold 10 gap in the centromeric region 5 Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g Os11g asDDM1 - and TSA - specific recombination region Supplementary Fig. S2
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(a) (b) Supplementary Fig. S3
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Supplementary Fig. S4 RM7463 YH1106 YH1105 YH1102 RM5857 YH1101 RM3428
400 kb recombination- repressed wt 3 recombination frequency (%) TSA 3 asDDM1 3 Supplementary Fig. S4
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