1. MCF-7: E2 E2(-4h)+OHT E2(-4h)+ICI 4 8 24 48 4 8 24 48 4 8 24 48 (h)4 8 24 48 4 8 24 48 4 8 24 48
(h)
FOXM1
ERα
pS2
PLK
CDC25b
Cyclin D1
Tubulin
Supplementary Figure S1. The induction of FOXM1 expression by E2 is antagonised by
OHT and ICI in MCF-7 cells.
MCF-7 cells were cultured in 5% double-charcoal striped FCS and phenol red free medium for 24h
before stimulated with E2 (-4h). Four hours after E2 restimulation the MCF-7 cells were also treated
with OHT or ICI. At times indicated, cells were collected and analysed for FOXM1, ERα, pS2,
CDC25b, cyclin D1 and tubulin expression by western blotting. The result showed that the ERα
targets pS2 and cyclin D1 and the FOXM1 targets PLK and CDC25b were induced by E2 and
repressed by OHT and ICI.
Millour et al Fig S1