1. CLINICAL PATHOLOGY: C-Reactive Protein
GENERAL OBJECTIVE:
After finishing lab activity of C-RP, the student will be able to describe C-RP in the DMS case problem
SPECIFIC OBJECTIVE:
At the end of lab activity of C-RP, the student will be able to interprete:
- the normal and abnormal C-RP

2. C-Reactive Protein
C-RP is a serum constituent originally define by its ability to precipitate Pneumococcus C polysaccharide
C-RP is an acute phase reactant:
A protein synthesized by the liver as a non-specific response to the inflammation

3. C-Reactive Protein
C-RP concentration increased in the serum of individu as a response to varies inflammatory condition and tissue necrosis
before specific antibody production starts and the ESR is increased.
After successful therapy, CRP level fall before the ESR decreases

4. C-Reactive Protein Specimen: serum Reference range:
1. < 6 mg/L (aglutination method),
semiquantitative
2. Immunoturbidimetric (quantitative)
< 5 mg/L

5. C-Reactive Protein Method: Latex agglutination Specimen: Serum
Principle:
Monoclonal anti C-RP will binds and agglutinate the CRP in the specimen

6. PROCEDURE
Allow each component of reagents and specimen to reach room temperature
Gently shake the reagent
Place a drop of undiluted specimen to the slide
Add one drop of the latex reagent next to the drops of specimen
Spread the reagent and specimen over theentire area of test circle
Gently tilt the test slide backwards and forwards for two minutes.
Positive and negative controls should be included

8. INTERPRETATION
Presence of agglutination indicates a level of C-RP in the specimen equal or > 6mg/L
The lack of agglutination indicates a C-RP level < 6 mg/L