1. by Carmen W. van den Berg, and Karolina E. Taylor
Letter Regarding Article by Li et al, “C-Reactive Protein Upregulates Complement-Inhibitory Factors in Endothelial Cells”
by Carmen W. van den Berg, and Karolina E. Taylor
Circulation
Volume 110(25):e542-e542
December 21, 2004
Copyright © American Heart Association, Inc. All rights reserved.

2. EA.hy926 cells were incubated for 24 h in medium without additional additives (−); with azide (Az; equivalent to 50 μg/mL CRP=0.0025% azide); Calbiochem CRP (C; 50 μg/mL), dialyzed Calbiochem CRP (C dial; 50 μg/mL); our recombinant azide-free CRP (O; 50 μg/mL); or our recombinant CRP with azide added (O + Az; % azide).
EA.hy926 cells were incubated for 24 h in medium without additional additives (−); with azide (Az; equivalent to 50 μg/mL CRP=0.0025% azide); Calbiochem CRP (C; 50 μg/mL), dialyzed Calbiochem CRP (C dial; 50 μg/mL); our recombinant azide-free CRP (O; 50 μg/mL); or our recombinant CRP with azide added (O + Az; % azide). DAF expression was assessed by flow cytometry. Results are expressed as median fluorescent intensity (MFI)±SD of experiments carried out in triplicate. Statistical analysis was carried out by one-way ANOVA followed by the Tukey multiple comparison test with 95% CI.
Carmen W. van den Berg, and Karolina E. Taylor Circulation. 2004;110:e542
Copyright © American Heart Association, Inc. All rights reserved.