1. GFP‐Sed5p localization defect in sgt2Δ.
GFP‐Sed5p localization defect in sgt2Δ. (A) Microscopy. GFP‐Sed5p localization in WT, sgt2Δ, mdy2Δ, and get3Δ strains demonstrating a defect in GFP‐Sed5p localization in sgt2Δ. These results support the placement of SGT2 in the tail‐anchored protein biogenesis pathway shown in Figure 3D. (B) Quantitative analysis. The images of at least 30 cells per strain with similar average fluorescence were quantified to determine the distribution of each strain's total fluorescence across pixels of different intensities. The distribution of fluorescence in the sgt2Δ strain differs from that of the wild‐type strain with P<1e−13, and is similar to the distribution for the knockout strains of other genes known to be involved in this pathway.
Alexis Battle et al. Mol Syst Biol 2010;6:379
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